Understanding the neurovascular coupling mechanisms and its neural effects
| Project/Area Number |
25750400
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Basic / Social brain science
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| Research Institution | The University of Electro-Communications |
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Principal Investigator |
MASAMOTO KAZUTO 電気通信大学, 情報理工学(系)研究科, 准教授 (60455384)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 神経血管連関 / グリア / 蛍光グルコース誘導体 / レーザースペックル血流計 / 蛍光標識グルコース誘導体 / 光イメージング / 脳微小循環 / 血流計測 / 神経活動 / 糖代謝 |
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| Outline of Final Research Achievements |
We developed an in vivo imaging method for glucose transportation from blood circulation to brain cells using fluorescent glucose analogue and two-photon microscopy in the awake and anesthetized rodents. The fluorescent glucose was intravenously injected to the animals, while the three-dimensional fluorescent images of the brain cells and vasculatures were captured up to depths of 0.3 mm in the cortex. We found that the fluorescent glucose was slowly transferred to the tissue, and this transportation pathway differs between the awake and anesthetized animals. Our results indicate that the isoflurane anesthesia may open the blood-brain barrier, which enhance the tissue leakage of the intravenously-injected fluorescent glucose.
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Report
(3 results)
Research Products
(32 results)
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[Journal Article] Vessel specific imaging of glucose transfer with fluorescent glucose analogue in anesthetized mouse cortex.2014
Author(s)
Murata R, Takada Y, Takuwa H, Kawaguchi H, Ito H, Kanno I, Tottori N, Yamada Y, Tomita Y, Itoh Y, Suzuki N, Yamada K, Masamoto K.
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Journal Title
Adv Exp Med Biol
Volume: ８１２
Pages: 241-246
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Microvascular sprouting, extension, and creation of new capillary connections with adaptation of the neighboring astrocytes in adult mouse cortex under chronic hypoxia.2014
Author(s)
Masamoto K, Takuwa H, Seki C, Taniguchi J, Itoh Y, Tomita Y, Toriumi H, Unekawa M, Kawaguchi H, Ito H, Suzuki N, Kanno I.
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Journal Title
J Cereb Blood Flow Metab.
Volume: 34
Pages: 325-331
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Pial arteries respond earlier than penetrating arterioles to neural activation in the somatosensory cortex in awake mice exposed to chronic hypoxia: an additional mechanism to proximal integration signaling?2014
Author(s)
Yuta Sekiguchi, Hiroyuki Takuwa, Hiroshi Kawaguchi, Takahiro Kikuchi, Eiji Okada, Iwao Kanno, Hiroshi Ito, Yutaka Tomita, Yoshiaki Itoh, Norihiro Suzuki, Ryo Sudo, Kazuo Tanishita, Kazuto Masamoto
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Journal Title
J Cereb Blood Flow Metab
Volume: 34
Pages: 1761-1770
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Vessel specific imaging of glucose transfer with fluorescent glucose analogue in anesthetized mouse cortex.2014
Author(s)
Murata R, Takada Y, Takuwa H, Kawaguchi H, Ito H, Kanno I, Tottori N, Yamada Y, Tomita Y, Itoh Y, Suzuki N, Yamada K, Masamoto K.
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Journal Title
Adv Exp Med Biol.
Volume: 812
Pages: 241-246
DOI
ISBN
9781493905836, 9781493906208
Related Report
Peer Reviewed / Open Access
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[Journal Article] 34. Microvascular sprouting, extension, and creation of new capillary connection with adaptation of the neighboring astrocytes in adult mouse cortex under chronic hypoxia2014
Author(s)
Masamoto K, Takuwa H, Seki C, Taniguchi J, Itoh Y, Tomita Y, Toriumi H, Unekawa M, Kawaguchi H, Ito H, Suzuki N, Kanno I
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Journal Title
J Cereb Blood Flow Metab
Volume: 34
Issue: 2
Pages: 325-31
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Cellular-scale imaging of glucose transfer in cerebral microvasculature with fluorescent glucose analogue in anesthetized mouse cortex.2013
Author(s)
R. Murata, Y. Takada, H. Takuwa, H. Kawaguchi, H. Ito, I. Kanno, N. Tottori, Y. Yamada, Y. Tomita, Y. Itoh, N. Suzuki, K. Yamada, K. Masamoto.
Organizer
The 41st Annual meeting of ISOTT
Place of Presentation
Hanover, NH, USA
Related Report
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