| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In this research, we developed a label-free counting device for exosomes using diffracted light derived from nanospaces. For the detection of exosomes with 100 nm in diameter, we fabricated nanospaces (150 nm in width and height), or nanostructures (10-100 nm in diameter) embedded in microchannels. After that, we introduced biological samples (with and without exosomes), such as plasma, urine, and cell supernatant, into the microchannels, and we compared label-free signals. Finally, we could achieve highly sensitive label-free detection of exosomes.
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