Label-free exosome counting for early diagnosis of cancer

• Project/Area Number: 25790028
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nanobioscience
• Research Institution: Nagoya University
• Principal Investigator: YASUI Takao 名古屋大学, 工学(系)研究科(研究院), 助教 (00630584)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: エクソソーム / 非標識カウンティング / microRNA / がん早期診断 / 非標識カウンティングデバイス / miRNA

Outline of Final Research Achievements

In this research, we developed a label-free counting device for exosomes using diffracted light derived from nanospaces. For the detection of exosomes with 100 nm in diameter, we fabricated nanospaces (150 nm in width and height), or nanostructures (10-100 nm in diameter) embedded in microchannels. After that, we introduced biological samples (with and without exosomes), such as plasma, urine, and cell supernatant, into the microchannels, and we compared label-free signals. Finally, we could achieve highly sensitive label-free detection of exosomes.

Research Products

• [Journal Article] Arrangement of a Nanostructure Array To Control Equilibrium and Nonequilibrium Transports of Macromolecules (2015)
  • Author(s): Yasui, T. Kaji, N. Ogawa, R. Hashioka, S. Tokeshi, M. Horiike, Y. Baba, Y.
  • Journal Title: Nano Letters Volume: XXX Issue: 5 Pages: 3445-3451
  • DOI: 10.1021/acs.nanolett.5b00783
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Self-assembled Nanowire Arrays as Three-dimensional Nanopores for Filtration of DNA Molecules (2015)
  • Author(s): S. Rahong, T. Yasui, T. Yanagida, K. Nagashima, M. Kanai, G. Meng, Y. He, F. Zhuge, N. Kaji, T. Kawai, Y. Baba
  • Journal Title: Analytical Sciences Volume: 31 Issue: 3 Pages: 153-157
  • DOI: 10.2116/analsci.31.153
  • NAID: 130004827469
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access
• [Journal Article] Three-dimensional Nanowire Structures for Ultra-Fast Separation of DNA, Protein and RNA Molecules (2015)
  • Author(s): Rahong, S. Yasui, T. Yanagida, T. Nagashima, K. Kanai, M. Meng, G. He, Y. Zhuge, F. Kaji, N. Kawai, T. Baba, Y.
  • Journal Title: Scientific Reports Volume: XXX
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ultrafast and wide range analysis of DNA molecules using rigid network structure of solid nanowires (2014)
  • Author(s): S. Rahong, T. Yasui, T. Yanagida, K. Nagashima, M. Kanai, A. Klamchuen, G. Meng, Y. He, F. Zhuge, N. Kaji, T. Kawai, Y. Baba
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 5252-5252
  • DOI: 10.1038/srep05252
  • Peer Reviewed / Open Access
• [Journal Article] Nanopillar, nanowall, and nanowire devices for fast separation of biomolecules (2014)
  • Author(s): Yasui, T. Rahong, S. Kaji, N. Baba, Y.
  • Journal Title: Israel Journal of Chemistry Volume: 54 Issue: 11-12 Pages: 1556-1563
  • DOI: 10.1002/ijch.201400102
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Micropore channel-based simultaneous electrical and optical sensing from single biomolecules, single exosomes to single cells (2014)
  • Author(s): Yasaki, H.; Yasui, T.; Rahong, S.; Yanagida, T.; Kaji, N.; Kanai, M.; Nagashima, K.; Kawai, T.; Baba, Y
  • Journal Title: Micro Total Analysis Systems 2014 Volume: 1 Pages: 2161-2163
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Label-free detection and quantification of real-time DNA amplification using one-dimensional photonic crystal (2013)
  • Author(s): T. Yasui, K. Ogawa, N. Kaji, M. Nilsson, M. Tokeshi, Y. Horiike and Y. Baba
  • Journal Title: Micro Total Analysis Systems 2013 Volume: 1 Pages: 1123-1125
  • Peer Reviewed
• [Journal Article] Christmas-tree nanowire chips for DNA separation (2013)
  • Author(s): S. Rahong, T. Yasui, T. Yanagida, M. Kanai, K. Nagashima, A. Klamchuen, M. Gang, H. Yong, F. Zhuge, N. Kaji, Y. Baba and T. Kawai
  • Journal Title: Micro Total Analysis Systems 2013 Volume: 1 Pages: 164-166
  • Peer Reviewed
• [Journal Article] Nanowire devices for exosomal microRNA extraction (2013)
  • Author(s): S. Ito, T. Yasui, H. Yong, T. Yanagida, S. Rahong, M. Kanai, K. Nagashima, H. Yukawa, N. Kaji, T. Kawai and Y. Baba
  • Journal Title: Micro Total Analysis Systems 2013 Volume: 1 Pages: 1887-1889
  • Peer Reviewed
• [Presentation] ナノワイヤを用いた細胞外小胞体の解析 (2015)
  • Author(s): 安井隆雄 小中出侑樹 伊藤聡 柳田剛 加地範匡 金井真樹 長島一樹 川合知二 馬場嘉信
  • Organizer: 日本化学会 第95春季年会(2015)
  • Place of Presentation: 船橋・日本大学理工学部船橋キャンパス／薬学部
  • Year and Date: 2015-03-26
• [Presentation] DNA Extraction from Bacteria Using Nanowire Structures (2014)
  • Author(s): Yasui, T. Otsuka, K. Takeuchi, M. Yanagida, T. Kaji, N. Rahong, S. Kanai, M. Nagashima, K. Kawai, T. Baba, Y.
  • Organizer: 14th Asia-Pacific International Symposium on Microscale Separations and Analysis (APCE2014)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2014-12-09
• [Presentation] 菌体検出のためのナノワイヤデバイス (2014)
  • Author(s): 安井隆雄 大塚康平 竹内将城 柳田剛 加地範匡 Sakon Rahong 金井真樹 長島一樹 川合知二 馬場嘉信
  • Organizer: 第30回化学とマイクロ・ナノシステム研究会 (30th CHEMINAS)
  • Place of Presentation: 札幌、北海道大学
  • Year and Date: 2014-10-02
• [Presentation] ナノワイヤデバイスによるエクソソームmiRNA解析 (2014)
  • Author(s): 安井隆雄 伊藤聡 柳田剛 加地範匡 He Yong Sakon Rahong 金井真樹 長島一樹 渡慶次学 川合知二 馬場嘉信
  • Organizer: 日本分析化学会第63年会
  • Place of Presentation: 東広島、広島大学
  • Year and Date: 2014-09-19
• [Presentation] ナノワイヤ構造体を用いた生体分子解析 (2014)
  • Author(s): 安井隆雄
  • Organizer: 第33回分析化学中部夏期セミナー
  • Place of Presentation: 富山
  • Year and Date: 2014-09-05
  • Invited
• [Presentation] Nanowire devices for exosomal microRNA detection (2014)
  • Author(s): Yasui, T. Ito, S. Yong, H. Yanagida, T. Rahong, S. Kanai, M. Nagashima, K. Yukawa, H. Kaji, N. Kawai, T. Baba, Y.
  • Organizer: The 6th International Symposium on Microchemistry and Microsystems (ISMM 2014)
  • Place of Presentation: Singapore
  • Year and Date: 2014-07-31
• [Presentation] 尿中エクソソームmiRNA 解析のためのナノワイヤデバイス (2014)
  • Author(s): 安井隆雄 伊藤聡 柳田剛 加地範匡 Yong He Sakon Rahong 金井真樹 長島一樹 湯川博 川合知二 馬場嘉信
  • Organizer: 第29回化学とマイクロ・ナノシステム研究会 (29th CHEMINAS)
  • Place of Presentation: 東京、日本女子大学
  • Year and Date: 2014-05-22
• [Presentation] ナノ構造体を用いた生体分子解析 (2014)
  • Author(s): 安井隆雄
  • Organizer: 第29回化学とマイクロ・ナノシステム研究会 (29th CHEMINAS)
  • Place of Presentation: 東京、日本女子大学
  • Year and Date: 2014-05-22
  • Invited
• [Presentation] Nanowire devices for biomolecule analysis (2014)
  • Author(s): T. Yasui, T. Yanagida, N. Kaji, T. Kawai and Y. Baba
  • Organizer: Pittcon 2014
  • Place of Presentation: Chicago, IL
  • Invited
• [Presentation] ナノバイオデバイスを用いたCTC、エクソソーム、細菌、ウィルス内の生体分子分離・解析 (2013)
  • Author(s): 安井隆雄
  • Organizer: JMAC第61回ワーキンググループ会議
  • Place of Presentation: 東京
  • Invited
• [Presentation] ナノ構造体を用いたDNA増幅のリアルタイム無標識検出 (2013)
  • Author(s): 安井隆雄, 小川謙亮, 加地範匡, 渡慶次学, 堀池靖浩, M. Nilsson and 馬場嘉信
  • Organizer: 第73回分析化学討論会(2013)
  • Place of Presentation: 函館・北海道大学
• [Presentation] Label-free detection and quantification of real-time DNA amplification using one-dimensional photonic crystal (2013)
  • Author(s): T. Yasui, K. Ogawa, N. Kaji, M. Nilsson, M. Tokeshi, Y. Horiike and Y. Baba
  • Organizer: The 17th international conference on miniaturized systems for chemistry and life sciences (uTAS2013)
  • Place of Presentation: Freiburg, Germany
• [Presentation] 3D Network Nanowires for DNA Separation (2013)
  • Author(s): T. Yasui, S. Rahong, T. Yanagida, N. Kaji, M. Kanai, K. Nagashima, A.Klamchuen, M. Gang, H. Yong, F. Zhuge, T. Kawai and Y. Baba
  • Organizer: Lab on a Chip Asia
  • Place of Presentation: Singapore
• [Patent(Industrial Property Rights)] 電気測定用チップ、及び電気測定装置 (2014)
  • Inventor(s): 矢崎啓寿 安井隆雄 加地範匡 馬場嘉信 柳田剛 川合知二
  • Industrial Property Rights Holder: 名古屋大学、大阪大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-214090
  • Filing Date: 2014-10-20
• [Patent(Industrial Property Rights)] 生体分子抽出用チップ、及び生体分子抽出用チップの製造方法 (2014)
  • Inventor(s): 安井隆雄 柳田剛 加地範匡 川合知二 馬場嘉信
  • Industrial Property Rights Holder: 名古屋大学、大阪大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-049054
  • Filing Date: 2014-03-12
• [Patent(Industrial Property Rights)] サンプルの破砕方法、生体分子の抽出方法、及びナノワイヤを含む破砕液 (2013)
  • Inventor(s): 安井隆雄 柳田剛 加地範匡 川合知二 馬場嘉信
  • Industrial Property Rights Holder: 名古屋大学、大阪大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-268424
  • Filing Date: 2013-12-26