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Single-molecule studies of circular DNA using nanomicrofluidic device in real-time

Research Project

Project/Area Number 25790030
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Nanobioscience
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Hirano Ken  国立研究開発法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (80392653)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords1分子 / DNA / 環状DNA / 1分子解析 / ナノ流体チップ / マイクロ流体チップ / リアルタイム / 1分子イメージング / 1分子DNA / ナノチップ
Outline of Final Research Achievements

In this study, we developed a nano- and microfluidic chip in order to perform the imaging of single circular DNA molecules directly in real time for dynamics studies based on biochemical reactions, the surrounding environment effect and thermodynamics. To observe the single circular DNA molecules directly ,we success the technique for imaging and measuring in real time with developing nano- and microfluidic chip. By using the chip, for instance, the condensation of single circular DNA molecules and single-strand-damage of the DNA in real time were able to measure and image in high sensitivity.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2015 2013 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Invited: 1 results)

  • [Journal Article] Marked difference in conformational fluctuation between giant DNA molecules in circular and linear forms2015

    • Author(s)
      Takafumi Iwaki, Tomomi Ishido, Ken Hirano, Alexei Lazutin, Valentina V. Vasilevskaya, Takahiro Kenmotsu and Kenichi Yoshikawa
    • Journal Title

      Journal of Chemical Physics

      Volume: 142 Issue: 14 Pages: 145101-145101

    • DOI

      10.1063/1.4916309

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Plasmonic Imaging of Brownian Motion of Single DNA Molecules Spontaneously Binding to Ag Nanoparticles2013

    • Author(s)
      平野 研、石堂 智美、山本 裕子、村瀬 至生、市川 正敏、吉川 研一、馬場 嘉信、伊藤 民武
    • Journal Title

      NANO LETTERS

      Volume: 13 Issue: 5 Pages: 1877-1882

    • DOI

      10.1021/nl304247n

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] マイクロ流路を用いた1分子 DNA の凝縮転移のリアルタイム解析2015

    • Author(s)
      平野 研、市川正敏、吉川研一
    • Organizer
      第32回「センサ・マイクロマシンと応用システム」シンポジウム
    • Place of Presentation
      朱鷺メッセ(新潟市)
    • Year and Date
      2015-10-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 直接観察による環状DNA1分子における凝縮過程の解析

    • Author(s)
      石堂智美、市川正敏、脇田慎一、吉川研一、平野 研
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場
    • Related Report
      2013 Research-status Report
  • [Presentation] DNA1分子のリアルタイムイメージングと操作・解析

    • Author(s)
      平野 研
    • Organizer
      2014年度第一回静電気学会研究会
    • Place of Presentation
      東京工業大学大岡山キャンパス
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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