Single-molecule studies of circular DNA using nanomicrofluidic device in real-time
| Project/Area Number |
25790030
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanobioscience
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Hirano Ken 国立研究開発法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (80392653)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | １分子 / DNA / 環状DNA / １分子解析 / ナノ流体チップ / マイクロ流体チップ / リアルタイム / １分子イメージング / １分子DNA / ナノチップ |
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| Outline of Final Research Achievements |
In this study, we developed a nano- and microfluidic chip in order to perform the imaging of single circular DNA molecules directly in real time for dynamics studies based on biochemical reactions, the surrounding environment effect and thermodynamics. To observe the single circular DNA molecules directly ,we success the technique for imaging and measuring in real time with developing nano- and microfluidic chip. By using the chip, for instance, the condensation of single circular DNA molecules and single-strand-damage of the DNA in real time were able to measure and image in high sensitivity.
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Report
(4 results)
Research Products
(5 results)
