Hydrogel-based immunocapture sheet for in vitro evaluation of contraction-dependent myokine secretion in skeletal muscle cells
Project/Area Number |
25790031
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Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Nano/Microsystems
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Research Institution | Tohoku University |
Principal Investigator |
NAGAMINE Kuniaki 東北大学, 工学(系)研究科(研究院), 助教 (00551540)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | ハイドロゲル / 骨格筋 / マイオカイン / 筋肉細胞アッセイデバイス / ウエット接着 / 骨格筋細胞 / センサ |
Outline of Final Research Achievements |
Hydrogel-based immunocapture sheet for in situ detection of myokines secreted from contracting skeletal muscle cell was developed to examine exercise-dependent metabolic regulation. For stable lamination of the immunocapture sheet on wet cell or tissue surface, fibrin-based biocompatible glue was also examined, and showed stable bonding of wet cationic and anionic hydrogels. The soft, molecularly permeable hydrogel-based immunocapture sheet was directly laminated to the skeletal muscle cells and sidplayed usefully measureable changes in myokine secretion of the muscle cells upon electrically-induced contraction. This hydrogel-based bioassay sheet will allow in vitro study of myokine-dependent regulation of glycemic homeostasis in the body, and will help us to understand the mechanism of exercise therapy for type-2 diabetes.
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Report
(3 results)
Research Products
(9 results)