| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Hydrogel-based immunocapture sheet for in situ detection of myokines secreted from contracting skeletal muscle cell was developed to examine exercise-dependent metabolic regulation. For stable lamination of the immunocapture sheet on wet cell or tissue surface, fibrin-based biocompatible glue was also examined, and showed stable bonding of wet cationic and anionic hydrogels. The soft, molecularly permeable hydrogel-based immunocapture sheet was directly laminated to the skeletal muscle cells and sidplayed usefully measureable changes in myokine secretion of the muscle cells upon electrically-induced contraction. This hydrogel-based bioassay sheet will allow in vitro study of myokine-dependent regulation of glycemic homeostasis in the body, and will help us to understand the mechanism of exercise therapy for type-2 diabetes.
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