| Project/Area Number |
25790034
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nano/Microsystems
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Research Collaborator |
WASHIZU Masao 東京大学, 工学系研究科, 教授 (10201162)
OANA Hidehiro 東京大学, 工学系研究科, 准教授 (20314172)
YANARU Rina 東京大学, 工学系研究科
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 細胞接着制御 / メッシュ培養法 / 細胞シート / 血管ー神経モデル / 共培養システム / 物質輸送計測 / マイクロデバイス / 細胞・基板相互作用 / 細胞シートの重層化 / 体外モデル / 細胞間相互作用 / 微細構造メッシュシート |
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| Outline of Final Research Achievements |
Cell-cell interactions and cross-boundary transport of essential substances are important for the functioning of most body tissues. In order to measure cell-cell interaction and transport at the blood-nerve interface, we developed a novel technology for fabricating free standing cell sheets where cells are cultured on microfabricated mesh sheets set suspended in a culture medium. The mesh sheets have narrow mesh strands (3-5 μm in width) for cell adhesion and large apertures (100-200 μm in pitch) such that when suspended, cells cultured on them grow under the condition of highly minimized adhesion area. Minimization of cell-substrate interaction results in an increase in cell-cell adhesion and induces self-assembly organization of cells into cell sheets. We are currently using this method to develop cell sheets of epithelial and endothelial blood cells that will be stacked to create a blood vessel model for eventual integration with nerve cells to realize a blood-nerve system model.
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