| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
This project is a proof-of concept study of rapid, sensitive, and on-site detection of bacteria using DNA amplidication on an electrode-integrated microfluidic device. Concentration of samples under alternating electric fields in a microfluidic channel was demonstrated. DNA detection by rolling circle amplification (RCA) was also demonstrated. Poly(ethylene glycol) (PEG) was used to facilitate RCA by molecular crowding effects. The number of RCA products increased in the presence of PEG.
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