Rapid, sensitive, and on-site detection of bacteria using DNA amplidication on an electrode-integrated microfluidic device
| Project/Area Number |
25790037
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nano/Microsystems
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| Research Institution | Toyo University |
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Principal Investigator |
SASAKI Naoki 東洋大学, 理工学部, 講師 (30462691)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | RCA / マイクロ流路 / 分子クラウディング / マイクロ電極 / 交流電場 / マイクロデバイス / DNA増幅 |
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| Outline of Final Research Achievements |
This project is a proof-of concept study of rapid, sensitive, and on-site detection of bacteria using DNA amplidication on an electrode-integrated microfluidic device. Concentration of samples under alternating electric fields in a microfluidic channel was demonstrated. DNA detection by rolling circle amplification (RCA) was also demonstrated. Poly(ethylene glycol) (PEG) was used to facilitate RCA by molecular crowding effects. The number of RCA products increased in the presence of PEG.
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Report
(3 results)
Research Products
(9 results)
