Fiber-optic biosensor for sensitive detection of sorbitol-induced diabetic complications

• Project/Area Number: 25790070
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Optical engineering, Photon science
• Research Institution: Tokyo Metropolitan Industrial Technology Research Institute
• Principal Investigator: GESSEI Tomoko 地方独立行政法人東京都立産業技術研究センター, 開発本部 開発第二部 バイオ応用技術グループ, 副主任研究員 (80520220)
• Research Collaborator: MITSUBAYASHI Kohji ; KUDO Hiroyuki ; ARAKAWA Takahiro ; MONKAWA Akira ; TAKIMOTO Yuki
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: バイオセンサ / ソルビトール / 糖尿病合併症 / 光ファイバ / 蛍光検出 / 酵素サイクリング

Outline of Final Research Achievements

The sorbitol is known as an effective biomarker of diabetes complications by previous studies. The sorbitol sensor with simple, compact and relatively accurate is required for daily prevention of diabetic complications. We constructed and tested fiber-optic biosensor for sensitive detection of sorbitol. The result gained from the measurement of sorbitol indicates that calibration of sorbitol in concentration ranges from 1.0 to 1000 μmol/l. This range also includes the standard sorbitol value in urine and blood for diabetic complication diagnosis.

Research Products

• [Journal Article] A fiber-optic sorbitol biosensor based on NADH fluorescence detection toward rapid diagnosis of diabetic complications (2015)
  • Author(s): Tomoko Gessei, Takahiro Arakawa, Hiroyuki Kudo and Kohji Mitsubayashi
  • Journal Title: Analyst Volume: 140 Issue: 18 Pages: 6335-6342
  • DOI: 10.1039/c4an01593b
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Fiber-optic biosensor with a UV-LED excitation system for sensitive determination of sorbitol-induced diabetic complications (2015)
  • Author(s): Tomoko Gessei, Yuki Takimoto, Akira Monkawa, Takahiro Arakawa, Kohji Mitsubayashi
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2015
  • Place of Presentation: Hawaii Convention Center
  • Year and Date: 2015-12-15
  • Int'l Joint Research