Creation of crystalline nanocavities based on peptide folding

• Project/Area Number: 25810046
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional solid state chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: SAWADA Tomohisa 東京大学, 工学(系)研究科(研究院), 助教 (70625467)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 自己組織化 / 結晶材料 / ペプチド / 配位結合 / 分子認識 / ナノ空間 / フォールディング / 単結晶

Outline of Final Research Achievements

In this work, a new artificial peptide-based nanomaterial was created by the strategy of coordination-driven self-assembly with concomitant folding and assembly processes. A tripeptide with coordinating groups at both termini was designed and successfully complexed with silver(I) ion to give a crystalline porous material. The nanochannel of this material was composed of polyproline II helix of the peptide ligand and its diameter reached more than 2-nm-size. Furthermore, unique molecular recognition properties of this new material were also revealed.

Research Products

• [Journal Article] Coordination-Driven Folding and Assembly of a Short Peptide into a Protein-like Two-Nanometer-Sized Channel (2014)
  • Author(s): Tomohisa Sawada, Asami Matsumoto, Makoto Fujita
  • Journal Title: Angew. Chem. Int. Ed. Volume: 53 Issue: 28 Pages: 7228-7232
  • DOI: 10.1002/anie.201403506
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mutual Induced Fit in a Synthetic Host-Guest System (2014)
  • Author(s): Tomohisa Sawada, Hayato Hisada, Makoto Fujita
  • Journal Title: J. Am. Chem. Soc. Volume: 136 Issue: 12 Pages: 4449-4451
  • DOI: 10.1021/ja500376x
  • Peer Reviewed
• [Presentation] 結晶性ペプチドらせんナノ空間を用いた親水性高分子の認識 (2015)
  • Author(s): 宮路達貴、澤田知久、藤田 誠
  • Organizer: 日本化学会第95回春季年会
  • Place of Presentation: 日本大学（千葉）
  • Year and Date: 2015-03-29
• [Presentation] D-アミノ酸を利用したペプチドの特異配座の創出 (2015)
  • Author(s): 足立精宏、澤田知久、藤田 誠
  • Organizer: 日本化学会第95回春季年会
  • Place of Presentation: 日本大学（千葉）
  • Year and Date: 2015-03-28
• [Presentation] βシート型ペプチドを基盤とする結晶性配位ナノチューブの構築 (2015)
  • Author(s): 大坪裕介、澤田知久、藤田 誠
  • Organizer: 日本化学会第95回春季年会
  • Place of Presentation: 日本大学（千葉）
  • Year and Date: 2015-03-28
• [Presentation] プロリン配列ペプチドの配位結合駆動フォールディング・アセンブリー (2015)
  • Author(s): 澤田知久、松本麻美、山上樹也、藤田 誠
  • Organizer: 日本化学会第95回春季年会
  • Place of Presentation: 日本大学（千葉）
  • Year and Date: 2015-03-27
• [Presentation] ポリプロリンIIヘリックスを基盤とする結晶性配位ネットワークの構造制御 (2015)
  • Author(s): 山上樹也、澤田知久、藤田 誠
  • Organizer: 日本化学会第95回春季年会
  • Place of Presentation: 日本大学（千葉）
  • Year and Date: 2015-03-26
• [Presentation] Creation of de novo peptide-based nanostructures by coordination-driven self-assembly (2015)
  • Author(s): Yusuke Otsubo, Tomohisa Sawada, Makoto Fujita
  • Organizer: The 3rd International Symposium "Dynamical ordering of biomolecular systems for creation of integrated functions"
  • Place of Presentation: 合歓の郷（三重）
  • Year and Date: 2015-01-10
• [Presentation] 短いペプチドヘリックスやシートを基盤とする配位ナノ構造形成 (2014)
  • Author(s): 大坪裕介、澤田知久、藤田 誠
  • Organizer: 第15回リング・チューブ超分子研究会シンポジウム
  • Place of Presentation: 東京工業大学（東京）
  • Year and Date: 2014-10-27
• [Presentation] (L-D-L-L)n周期配列ペプチドの配座探索 (2014)
  • Author(s): 足立精宏、澤田知久、藤田 誠
  • Organizer: 第4回CSJフェスタ2014
  • Place of Presentation: タワーホール船堀（東京）
  • Year and Date: 2014-10-15
• [Presentation] A Crystalline Folded Peptide Coordination Network with Protein-Like Two-Nanometer-Sized Channles (2014)
  • Author(s): Tomohisa Sawada, Asami Matsumoto, Makoto Fujita
  • Organizer: 4th International Conference on Metal-Organic Frameworks and Open Framework Compounds
  • Place of Presentation: 神戸国際会議場（兵庫）
  • Year and Date: 2014-09-29
• [Presentation] 人工配位性ペプチドによる精密ナノ構造構築 (2014)
  • Author(s): 大坪裕介、澤田知久、藤田 誠
  • Organizer: 第64回錯体化学討論会
  • Place of Presentation: 中央大学（東京）
  • Year and Date: 2014-09-19
• [Presentation] Creation of Crystalline Peptide Helical Nanochannels through Coordination-Driven Folding and Assembly (2014)
  • Author(s): 澤田知久、松本麻美、藤田 誠
  • Organizer: 第64回錯体化学討論会
  • Place of Presentation: 中央大学（東京）
  • Year and Date: 2014-09-18
• [Presentation] A Crystalline Folded Peptide Coordination Network with Nanometer-Size Channels
  • Author(s): Tomohisa Sawada, Asami Matsumoto, Makoto Fujita
  • Organizer: 8th International Symposium on Macrocyclic and Supramolecular Chemistry
  • Place of Presentation: Crystal Gateway Marriott, Virginia, USA
• [Presentation] 相互誘導適合によるホスト･イン･ホスト錯体の構築
  • Author(s): 澤田知久、久田隼人、藤田誠
  • Organizer: 錯体化学会第６３回討論会
  • Place of Presentation: 琉球大学（沖縄県）
• [Presentation] ２ナノメートル径のチャネルを有する結晶性ペプチドフォールド配位ネットワークの創出
  • Author(s): 松本麻美、澤田知久、藤田誠
  • Organizer: 錯体化学会第６３回討論会
  • Place of Presentation: 琉球大学（沖縄県）
• [Presentation] 人工系における相互誘導適合：ホスト･イン･ホスト型分子の構築
  • Author(s): 澤田知久、久田隼人、藤田誠
  • Organizer: 第９４回日本化学会春季年会
  • Place of Presentation: 名古屋大学（愛知県）
• [Presentation] 結晶性ペプチドフォールド配位ネットワークの創出とその分子認識
  • Author(s): 松本麻美、澤田知久、藤田誠
  • Organizer: 第９４回日本化学会春季年会
  • Place of Presentation: 名古屋大学（愛知県）