| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
A fluorescent silylated rare earth metal complex was prepared by a very simple procedure through formation of a Schiff base between a rare earth metal complex bearing a formyl group and 3-aminopropyltriethoxysilane. Using the silylated rare earth metal complex, silica nanoparticles modified chemically with rare earth metal complexes (R-SNPs) were synthesized by a reverse micelle method with Triton X-100 as a surfactant in cyclohexane. The fluorescence properties of rare earth metal complexes immobilized in silica nanoparticles were remarkably improved by photostability, pH dependence and fluorescence lifetime, compared to free rare earth metal complexes. Also, R-SNPs showed excellent dispersibility in aqueous solution and they hardly aggregated even under pseudo-psychological conditions. Furthermore, R-SNPs was usable as a label agent for the fluorescent imaging of African green monkey kidney cells.
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