| Project/Area Number |
25810102
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Toshikazu Ono 九州大学, 工学(系)研究科(研究院), 助教 (20643513)
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| Co-Investigator(Renkei-kenkyūsha) |
HISAEDA Yoshio 九州大学, 大学院工学研究院, 教授 (79777251)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ナノ材料 / エキシマー / エキシプレックス / センサー / DNA修復 / ソフトマテリアル / 蛍光 / オリゴDNA / 蛍光プローブ / 人工核酸 / 損傷塩基 / エネルギー移動 |
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| Outline of Final Research Achievements |
We report the development of simple fluorogenic probes that report on the activity of uracil DNA glycosylase (UDG) enzymes. The probes are built from short, modified single-stranded oligonucleotides containing natural and unnatural bases. The combination of multiple fluorescent pyrene and/or quinacridone nucleobases yields fluorescence at 480 and 540 nm (excitation 340 nm), with large Stokes shifts of 140 to 200 nm. They are strongly quenched by uracil bases incorporated into the sequence, and they yield light-up signals of up to 40-fold, or ratiometric signals with ratio changes of 82-fold, on enzymatic removal of these quenching uracils. The use of similar concepts, we report the development of simple fluorogenic probes that report on the activity of 8-oxoguanine glycosylase.
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