Evaluation of food webs and treatment mechanism in wastewater treatment systems using carbon and nitrogen stable isotope ratios

• Project/Area Number: 25820256
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Civil and environmental engineering
• Research Institution: National Institute for Environmental Studies
• Principal Investigator: ONODERA Takashi 独立行政法人国立環境研究所, 地域環境研究センター, 研究員 (30583356)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 炭素・窒素安定同位体比 / 下水処理 / 高次生物 / 微生物生態系

Outline of Final Research Achievements

The study conducted the evaluation of pray-predator relationship and treatment characteristics based on carbon and nitrogen stable isotope ratios so as to reveal the microbial ecosystem and to enhance the process performance in biological wastewater treatment systems. As a result, δ13C and δ15N of the retained sludge and associated organisms changed drastically within the system. Therefore δ13C and δ15N reflected both treatment and dietary characteristics. We concluded that δ13C and δ15N values are potentially useful as alternative indicators for investigating microbial ecosystems and treatment characteristics of biological wastewater treatment systems.

Research Products

• [Journal Article] Spatial changes in carbon and nitrogen stable isotope ratios of sludge and associated organisms in a biological sewage treatment system (2015)
  • Author(s): Onodera, T., Kanaya, G., Syutsubo, K., Miyaoka, Y., Hatamoto M., Yamaguchi, T.
  • Journal Title: Water Research Volume: 68 Pages: 387-393
  • DOI: 10.1016/j.watres.2014.10.020
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Evaluation of microbial and treatment characteristics in a sewage treatment system based on carbon and nitrogen stable isotope ratios (2014)
  • Author(s): Onodera, T., Kanaya, G., Syutsubo, K., Miyaoka, Y., Hatamoto M., Yamaguchi, T.
  • Organizer: IWA World Water Congress 2014
  • Place of Presentation: Lisbon (Portugal)
  • Year and Date: 2014-09-23
• [Presentation] Evaluation of microbial characteristics In a sewage treatment system based on stable isotope ratios (2014)
  • Author(s): T. Onodera, G. Kanaya, K. Syutsubo, Y. Miyaoka, M. Hatamoto, T. Yamaguchi
  • Organizer: IWA World Water Congress & Exhibition
  • Place of Presentation: Lisbon, Portugal
• [Presentation] 下水処理リアクターにおける炭素・窒素安定同位体比の空間的・微生物的分布の解析
  • Author(s): 小野寺崇，金谷弦，珠坪一晃，宮岡佑馬，幡本将史，山口隆司
  • Organizer: 第50回環境工学研究フォーラム
  • Place of Presentation: 札幌
• [Remarks] 研究紹介＞炭素・窒素安定同位体比を指標とした排水処理システムにおける浄化機構と食物網の解析
  • URL: http://www.nies.go.jp/subjects/22720_fy2014.html