Development of non-invasive cell cycle synchonization method by microfluidic devices
Project/Area Number |
25820397
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Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Biofunction/Bioprocess
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Research Institution | Yamagata University |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | マイクロデバイス / 分離操作 / 細胞周期 |
Outline of Final Research Achievements |
Cell cycle synchronization technique which is size-based sorting with microfluidic device was developed. Recovery efficiency of cells from the device was improved by albumin treatment in the microchannel. The recovery rate of G0/G1 phase and G2/M phase were over 90% and 50%, respectively. The size-based microfluidic device was able to separate the cells on G0/G1 phase efficiently by albumin treatment. Also, cell separation technique based on the attachment activity of each cell cycle phase was developed. G2/M phase and S phase may recover efficiently using this technique.
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Report
(4 results)
Research Products
(6 results)