| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Cell cycle synchronization technique which is size-based sorting with microfluidic device was developed. Recovery efficiency of cells from the device was improved by albumin treatment in the microchannel. The recovery rate of G0/G1 phase and G2/M phase were over 90% and 50%, respectively. The size-based microfluidic device was able to separate the cells on G0/G1 phase efficiently by albumin treatment.
Also, cell separation technique based on the attachment activity of each cell cycle phase was developed. G2/M phase and S phase may recover efficiently using this technique.
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