| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The central carbon metabolism in baker's yeast, Saccharomyces cerevisiae, was expanded by introducing metabolic pathways of other microorganisms to improve a capability of the yeast metabolism for more efficient bioproduction of useful chemicals. The metabolically engineered S. cerevisiae strains expressing bacterial Entner-Doudrouf and phosphoenolpyruvate carboxylase pathways were constructed whose functions were confirmed by the labeling experiment and isobutanol fermentaion test.
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