Assessment of deviation from the undifferentiated state in colonies of hiPSCs and development of its maintenance culture process
| Project/Area Number |
25820401
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University |
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Principal Investigator |
KIM MEE-HAE 大阪大学, 工学(系)研究科(研究院), 助教 (30506449)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | バイオ生産プロセス / 再生医療 / ヒトiPS細胞 / 未分化維持 / 生物・生体工学 / 細胞・組織 / 培養面設計 / iPS細胞 |
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| Outline of Final Research Achievements |
In this study, we describe an understanding of the possible mechanisms that control hiPSC self-renewal in cell culture conditions in the presence of feeder cells. From the standpoint of the deviation from the undifferentiated state in colonies of hiPSCs on feeder layers, the most important aspects for cell migration and growth inside a single colony are a balance between cell-cell and cell-substrate interactions that induces spatial differences in their morphological and functional characteristics. This approach can be applied to an important generic technology for expanding the in the undifferentiated state of hiPSCs.
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Report
(3 results)
Research Products
(14 results)
