| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We have designed LEA peptide based on the motif sequence of LEA protein from polypedilum vanderplanki, and the protein expression system was constructed to co-express target protein with the LEA peptide in E.coli. The purpose of the study is to construct and characterization of LEA peptide 13mer motif for the expression of target protein in E.coli, through the co-expression system. In this study we employed the mutation in our original LEA peptide sequence and replace the Glycine with other amino acids at the 6th and 12th position. The protein expression increased with time in LEA-K and LEA-N was co-expressed compared with co-expression with original LEA peptide. In contrast, the expression of GFP was reduced in LEA-S as compared to original one. We have also investigated enhancement of protein expression using LEA peptide co-expression system to control expression levels of the peptide. The expression of a target protein was more than ten times to co-express with the LEA peptide.
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