| Project/Area Number |
25820406
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
FUKUDA Nobuo 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (00613548)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 酵母 / 交雑育種 / 自己倍数化 / 増殖制御 / 接合 |
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| Outline of Final Research Achievements |
To breed superior yeast strains by the crossbreeding method, we need a-type and α-type of cells derived from two kinds of parental strains. Because both a-type and α-type of cells can derive from a single parental strain and mate with each other, autopolyploidization impedes isolation of the desired mating-type of cells and subsequent crossbreeding. Here, we designed a method to prevent autopolyploidization, aiming at facilitation of yeast crossbreeding. In the current study, we converted the mating-type of yeast cells by using chromosomal aberration during mitotic division. We succeeded in isolation of a-type or α-type of cells from cell mixture by preventing autopolyploidization. Our approach can be applied to industrial strains, and help promote further advance in yeast crossbreeding.
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