| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
We have developed an assessment method employing oligonucleotide samples consisting of several dozen base pairs and subjected to fluorescence modification, with a fluorescent image analyzer or fluorescent microscope used to measure the amount of radiation-induced damage. In this study, we exposed a oligonucleotide with helium ion beam and gamma rays of dose from 10 to 1000mGy. This study shows the radiation damage on the oligonucleotide can be directly detected with fluorescence spectrophotometer, but the fluorescence intensity nearly saturated when we applied more than 50 mGy. Since the fluorescents molecule may disintegrate by radiation, we also studied the radiolysis of the fluorescence molecules by helium ion beam and gamma rays.
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