Associative memory between object and place in hippocampus using virtual reality and in vivo two-photon calcium imaging

• Project/Area Number: 25830023
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MIZUTA KOTARO 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (60632891)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: トランスジェニックマウス / 二光子レーザー顕微鏡 / 可塑性 / 記憶・学習 / 場所細胞 / 報酬 / 蛍光カルシウムバイオセンサー / 神経可塑性 / 海馬

Outline of Final Research Achievements

We aimed to elucidate activity of cell assembly in hippocampus during association of object and place information. For the purpose, we imaged neuronal activities during the learning using transgenic mice that express a fluorescent calcium sensor protein G-CaMP7 in hippocampal CA1 pyramidal neurons. Mice stayed the correct target of three targets for 2 sec to receive reward in a virtual linear track. While the animals navigate through the track, a population of neurons exhibited place-cell like activity. In addition, another group of neurons demonstrated time-locked activity while the mice stayed at the target zone. The representation of target was stable: some of them remained multiple days and still responded even reward zone were relocated. These results indicate that information with higher value is more stably represented in subpopulations of neurons in hippocampal CA1 than that without value.

Research Products

• [Presentation] 海馬CA1における価値を持つ場所の選択的表現 (2015)
  • Author(s): 水田恒太郎、佐藤正晃、関根友紀子、河野真子、イスラムタンビル、増本年男、大倉正道、中井淳一、林康紀
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫、神戸）
  • Year and Date: 2015-07-28 – 2015-07-31
• [Presentation] 海馬-嗅内皮質の情報処理と移動ロボットのナビゲーション問題との関連性調査とモデル化 (2015)
  • Author(s): 上田隆一、水田恒太郎、山川宏、岡田浩之
  • Organizer: 第29回人工知能学会全国大会
  • Place of Presentation: 公立はこだて未来大学（北海道、函館）
  • Year and Date: 2015-05-31 – 2015-06-02
• [Presentation] 海馬CA1における価値表現の選択的保持 (2015)
  • Author(s): 水田恒太郎、佐藤正晃、関根友紀子、河野真子、イスラムタンビル、大倉正道、中井淳一、林康紀
  • Organizer: 第92回日本生理学会
  • Place of Presentation: 神戸国際会議場（兵庫、神戸）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Two-photon calcium imaging of hippocampal CA1 circuit activity during virtual spatial memory task (2014)
  • Author(s): Kotaro Mizuta, Masaaki Sato, Masako Kawano, Yukiko Sekine, Tanvir Isram, Masamichi Ohkura, Junichi Nakai, Yasunori Hayashi
  • Organizer: 生理学研究所「シナプス・神経ネットワークの機能ダイナミクス」
  • Place of Presentation: 生理学研究所（愛知、岡崎）
  • Year and Date: 2014-12-12 – 2014-12-13
• [Presentation] Two-photon calcium imaging of hippocampal CA1 circuit activity during virtual spatial memory task (2014)
  • Author(s): Kotaro Mizuta, Masaaki Sato, Masako Kawano, Yukiko Sekine, Tanvir Isram, Masamichi Ohkura, Junichi Nakai, Yasunori Hayashi
  • Organizer: Cold Spring Harbor Asia Conference, Neural Circuit Basis of Behavior and its Disorders
  • Place of Presentation: Suzhou, China
  • Year and Date: 2014-05-12 – 2014-05-16
• [Presentation] Memory-guided behavior of mice in a virtual environment (2014)
  • Author(s): Kotaro Mizuta, Masaaki Sato, Masako Kawano, Yukiko Sekine, Masamichi Ohkura, Junichi Nakai, Yasunori Hayashi
  • Organizer: International Symposium ”New Frontier of Molecular Neuropathology 2014”
  • Place of Presentation: 東京
• [Presentation] バーチャルリアリティ直線路における記憶に基づいたマウスのナビゲーション行動 (2014)
  • Author(s): 水田恒太郎、佐藤正晃、河野真子、関根友紀子、大倉正道、中井純一、林康紀
  • Organizer: 第９１回日本生理学会大会
  • Place of Presentation: 鹿児島
• [Presentation] 生体における海馬神経回路活動の可視化 (2013)
  • Author(s): 佐藤正晃、水田恒太郎、河野真子、竹川高志、イスラム・タンビル、山川宏、山口陽子、深井朋樹、大倉正道、中井淳一、林康紀
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
• [Presentation] 仮想現実環境における記憶に基づく空間ナビゲーション課題 (2013)
  • Author(s): 水田恒太郎、佐藤正晃 河野真子、林康紀
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
• [Presentation] Hippocampal CA1 network dynamics during locomotion in virtual reality (2013)
  • Author(s): Masaaki Sato, Kotaro Mizuta, Masako Kawano, Takashi Takekawa, Tanvir Islam, Hiroshi Yamakawa, Yoko Yamaguchi, Tomoki Fukai, Masamichi Ohkura, Junichi Nakai, Yasunori Hayashi
  • Organizer: Society for Neuroscience 43rd annual meeting
  • Place of Presentation: 米国サンディエゴ
• [Remarks] 記憶メカニズム研究チーム
  • URL: http://www.riken.jp/research/labs/bsi/mem_mech/