Analysis of prion prpagation using optical tweezers

• Project/Area Number: 25830025
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Komi Yusuke 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (20565999)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 光ピンセット / タンパク質一分子 / 天然変性タンパク質

Outline of Final Research Achievements

I investigated the conformational space of a yeast prion protein Sup35NM using optical tweezers.Sup35NM S17R mutant had a part of common structure, but to different confomational space from Sup35NM wild type. Sup35NM S17R with EPEE mutant had similar confomational space to Sup35NM wild type.

Research Products

• [Presentation] 光ピンセット法を用いた、Sup35NMコンフォメーション構造の解析 (2015)
  • Author(s): Yusuke Komi Rodrigo Maillard Piere Rodriguez Bustamante Carlos Motomasa Tanaka
  • Organizer: 生物物理学会
  • Place of Presentation: 金沢大学（石川県・金沢市）
  • Year and Date: 2015-09-13 – 2015-09-15
  • Invited
• [Presentation] Single molecule analysis of conformational space of a yeast prion protein Sup35NM using opticl tweezers (2015)
  • Author(s): Yusuke Komi, Rodrigo Maillard, Piere Rodriguez, Carlos Bustamante, Motomasa Tanaka
  • Organizer: 日本生物物理学会
  • Place of Presentation: 金沢大学角間キャンパス・金沢市・石川県
  • Year and Date: 2015-09-13
  • Invited
• [Presentation] Single molecule studies on the conformational heterogeneity of Sup35NM structure (2013)
  • Author(s): Yusuke Komi, Maillard Rodrigo, Carlos Bustamante, Motomasa Tanaka
  • Organizer: 日本生物物理学会
  • Place of Presentation: 京都