The analysis of the mitophagy in Parkinson's disease with new autophagy probes.

Research Project

Project/Area Number	25830043
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Nerve anatomy/Neuropathology
Research Institution	Institute of Physical and Chemical Research
Principal Investigator	Katayama Hiroyuki 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (00415126)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	オートファジー / 蛍光タンパク質 / マイトファジー / パーキンソン病 / リソソーム
Outline of Final Research Achievements	In this study, I have produced new autophagy and mitophagy probes with the two fluorescent proteins. These were able to detect autophagy and mitophagy in mammalian cells, respectively. The signals of those remained after fixation. so, those are suitable for observing with fixed samples. Actually, this autophagy probe was able to detect the starvation induced autophagy signal in mouse liver sections prepared using perfusion fixation. These new probes would be good tools for autophagy and mitophagy research.

Report

(4 results)