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Intracellular signaling in neural circuit assembly.

Research Project

Project/Area Number 25830049
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neurochemistry/Neuropharmacology
Research InstitutionNagoya University

Principal Investigator

NAKAMUTA Shinichi  名古屋大学, 医学(系)研究科(研究院), 助教 (20647474)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords神経細胞 / 軸索 / 樹状突起 / 細胞接着 / 細胞外因子 / CaMKs / 軸索形成 / 神経栄養因子 / 接着因子 / TAG-1
Outline of Final Research Achievements

Neurons are highly polarized cells that have structurally distinct processes―the axons and dendrites― that differentiate from common immature neurites. Various extracellular and intracellular signals contribute to axon specification; however, the specific intracellular pathways whereby particular extracellular stimuli lead to axon specification remain to be delineated. From our research, we found that the TAG-1-mediated cell-to-cell interaction between the unpolarized multipolar cells and the pioneering axons regulates the polarization of multipolar cells partly through Rac1. Recently, we identified the Rho guanine nucleotide exchange factor H1 (GEF-H1/ARHGEF2) as one of the CaMKs substrates in hippocampal neurons. GEF-H1 can be sequestered in an inactive state on polymerized microtubules. We identified the GEF-H1 phosphorylation site in the N-terminal region. GEF-H1 N-terminal region regulate its binding towards microtubules, thereby regulating the GEF activity.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (4 results) Remarks (2 results)

  • [Journal Article] In vivo Screening for Substrates of Protein Kinase A Using a Combination of Proteomic Approaches and Pharmacological Modulation of Kinase Activity.2015

    • Author(s)
      Hamaguchi T, Nakamuta S, Funahashi Y, Takano T, Nishioka T, Shohag MH, Yura Y, Kaibuchi K, Amano M.
    • Journal Title

      Cell Struct Funct.

      Volume: 40 Pages: 1-12

    • NAID

      130004712922

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neuronal polarization in vivo: Growing in a complex environment.2014

    • Author(s)
      Funahashi Y, Namba T, Nakamuta S, Kaibuchi K.
    • Journal Title

      Curr Opin Neurobiol.

      Volume: 27 Pages: 215-223

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pioneering axons regulate neuronal polarization in the developing cerebral cortex.2014

    • Author(s)
      Namba T, Kibe Y, Funahashi Y, Nakamuta S, Takano T, Ueno T, Shimada A, Kozawa S, Okamoto M, Shimoda Y, Oda K, Wada Y, Masuda T, Sakakibara A, Igarashi M, Miyata T, Faivre-Sarrailh C, Takeuchi K, Kaibuchi K.
    • Journal Title

      Neuron

      Volume: 81(4) Pages: 814-829

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] ERK2-mediated phosphorylation of Par3 regulates neuronal polarization.2013

    • Author(s)
      Funahashi Y, Namba T, Fujisue S, Itoh N, Nakamuta S, Kato K, Shimada A, Xu C, Shan W, Nishioka T, Kaibuchi K.
    • Journal Title

      J Neurosci.

      Volume: 33(33) Pages: 13270-13285

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Proteomic screening for substrates of protein kinases activated by dopamine stimulation2014

    • Author(s)
      Shinichi Nakamuta, Taku Nagai, Tomoki Nishioka, Mutsuki Amano, Akinori Nishi and Kozo Kaibuchi
    • Organizer
      第57回 日本神経化学会大会
    • Place of Presentation
      奈良県文化会館、奈良県新公会堂 (奈良県奈良市)
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] Proteomic screening for substrates of protein kinases activated by dopamine stimulation2014

    • Author(s)
      Shinichi Nakamuta, Taku Nagai, Tomoki Nishioka, Mutsuki Amano, Akinori Nishi and Kozo Kaibuchi
    • Organizer
      Neuroscience 2014
    • Place of Presentation
      パシフィコ横浜 (神奈川県横浜市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Proteomic screening for calcium/calmodulin–dependent protein kinases substrates2013

    • Author(s)
      Shinichi Nakamuta, Ayano Matsushima, Sharmin Aktar, Tomoki Nishioka and Kozo Kaibuchi
    • Organizer
      Neuro 2013
    • Place of Presentation
      京都国際会議場(京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] Proteomic screening for substrates of protein kinases activated by dopamine stimulation2013

    • Author(s)
      Shinichi Nakamuta, Taku Nagai, Tomoki Nishioka, Mutsuki Amano, Akinori Nishi and Kozo Kaibuchi
    • Organizer
      Gordon Research Conferences (catecholamines)
    • Place of Presentation
      West Dover, USA
    • Related Report
      2013 Research-status Report
  • [Remarks] 名古屋大学大学院 医学系研究科 神経情報薬理学講座HP

    • URL

      http://www.med.nagoya-u.ac.jp/Yakuri/index.htm

    • Related Report
      2014 Annual Research Report
  • [Remarks] 名古屋大学大学院 医学研究科 神経情報薬理学講座 HP

    • URL

      http://www.med.nagoya-u.ac.jp/Yakuri/

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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