Generation of transgenic pigs expressing Fluorescent Ubiquitination-based Cell Cycle Indicator, Fucci.
Project/Area Number |
25830066
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Laboratory animal science
|
Research Institution | Meiji University |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | Fucci / 細胞周期 / 蛍光プローブ / ブタ / 体細胞核移植 / 遺伝子改変クローンブタ / 遺伝子組換え動物 |
Outline of Final Research Achievements |
This study aimed to create genetically modified pigs expressing Fucci, a fluorescent probe that allows the real-time visualization of the cell cycle in living cells, by using somatic cell nuclear transfer (SCNT). Porcine fetal fibroblasts introducing Fucci vector were successfully established as nuclear donor cell for SCNT. Cloned pigs were subsequently generated using the cell established through SCNT and their progeny were created by sexual reproduction. Fluorescence observation of the tissues and organs of the cloned pigs and their progenies did not confirm the systemic expression of Fucci, but confirmed Fucci expression in some of the skin, the heart and the skeletal muscles. These results demonstrated that transgene silencing occurred following SCNT. DNA methylation analysis revealed that the promoter sequences in Fucci transgene was highly methylated.
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Report
(3 results)
Research Products
(2 results)
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[Presentation] 細胞周期可視化蛍光プローブFucci導入トランスジェニック・クローンブタの作出2014
Author(s)
渡邊將人, 林田豪太, 松成ひとみ, 中野和明, 小林美里奈, 松村幸奈, 倉本桃子, 坂井理恵子, 浅野吉則, 内倉鮎子, 新井良和, 梅山一大, 長屋昌樹, 阪上‐沢野朝子, 宮脇敦史, 長嶋比呂志
Organizer
第107回日本繁殖生物学会大会
Place of Presentation
帯広
Year and Date
2014-08-21 – 2014-08-24
Related Report
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[Presentation] 細胞周期可視化蛍光プローブFucciを発現するブタ体細胞核移植胚の作出2013
Author(s)
林田豪太, 渡邊将人, 松成ひとみ, 中野和明, 金井貴博, 小林美里奈, 松村幸奈, 倉本桃子, 坂井理恵子, 浅野吉則, 内倉鮎子, 前原美樹, 新井良和, 梅山一大, 長屋昌樹, 沢野朝子, 宮脇敦史, 長嶋比呂志
Organizer
日本繁殖生物学会
Place of Presentation
東京農工大学(東京)
Related Report