Development of recurrence mouse brain tumor model using inducible iCSCs and identification of target molecules in recurrence tumor
| Project/Area Number |
25830088
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 神経幹細胞 / ＡＬＫキナーゼ / 脳腫瘍モデル / ALKキナーセ / ALKキナーゼ |
|---|
| Outline of Final Research Achievements |
The cancer stem cells (CSCs) are thought that the cause of local recurrence or distant organ metastasis following therapy. In this study, we have established the inducible mouse brain tumor model transplanting the genetically modified neural stem cells (NSCs) using Tet-On system. Inducible expression of activated-ALK could transform the NSCs in the presence of Dox treatment in mice. Next, we performed the exome sequence analysis to investigate the genetic aberration in the activated-ALK or -H-RAS expressing NSCs. Exome data showed that characteristic change of copy number in each chromosomes were observed in these cells. Using the inducible mouse brain tumor model, we would like to establish the recurrence mouse brain tumor model and identify the target molecules in recurrence tumors by analyzing copy number change.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] IGF2 Preserves Osteosarcoma Cell Survival by Creating an Autophagic State of Dormancy That Protects Cells against Chemotherapeutic Stress2014
Author(s)
Shimizu T, Sugihara E, Yamaguchi-Iwai S, Tamaki S, Koyama Y, Kamel W, Ueki A, Ishikawa T, Chiyoda T, Osuka S, Onishi N, Ikeda H, Kamei J, Matsuo K, Fukuchi Y, Nagai T, Toguchida J, Toyama Y, Muto A, Saya H
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Journal Title
Cancer Res
Volume: 74
Issue: 22
Pages: 6531-6541
DOI
Related Report
Peer Reviewed
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[Journal Article] Twist2 functions as a tumor suppressor in murine osteosarcoma cells.2013
Author(s)
Ishikawa T, Shimizu T, Ueki A, Yamaguchi S, Onishi N, Sugihara E, Kuninaka S, Miyamoto T, Morioka H, Nakayama R, Kobayashi E, Toyama Y, Mabuchi Y, Matsuzaki Y, Yamaguchi R, Miyano S and Saya H
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Journal Title
Cancer Sci
Volume: (in press)
Issue: 7
Pages: 880-888
DOI
Related Report
Peer Reviewed
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