Cell-to-cell communication between tumor cell and their microenvironment via exosomes
Project/Area Number |
25830089
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Tumor biology
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Research Institution | Tokyo Medical University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | がん微小環境 / エクソソーム / miRNA / 多発性骨髄腫 / 血管新生 / 低酸素環境 / 細胞間相互作用 |
Outline of Final Research Achievements |
The aim of this study is to clarify the role of tumor-derived exosome in their microenvironment using multiple myeloma cell lines which show continuous growth in vitro under chronically hypoxic conditions (hypoxia-resistant MM cells; HR-MM cells). We isolated the exosomes from HR-MM cells and assessed exosomal miRNA profiling using the TaqMan low-density array. Endothelial tube formation assay was used for validating function of MM cell-endothelial cell (HUVECs) communication. We found miR-135b was significantly up-regulated in exosomes from HR-MM cells compared to those in the parental cells. The exosomal miR-135b directly suppressed its target, factor inhibiting HIF-1 (FIH-1), in endothelial cells, and enhanced endothelial tube formation under hypoxia. HR-MM cell-derived exosomes could enhance density of CD31 positive endothelial cells in Matrigel plug. Suggesting that the exosomal miR-135b might therefore be one of promising target for controlling angiogenesis in MM.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Down-regulated microRNA-148b in circulating PBMCs in chronic myeloid leukemia patients with undetectable minimal residual disease: A possible molecular classifier to stop imatinib safely.2014
Author(s)
Ohyashiki, J. H., Ohtsuki, K., Mizoguchi, I., Yoshimoto, T., Katagiri, S., Umezu, T., and Ohyashiki, K.
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Journal Title
Drug Des. Devel. Ther.
Volume: 8
Pages: 1151-1159
DOI
Related Report
Peer Reviewed / Open Access
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