| Project/Area Number |
25830093
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
SHINTARO Fukushima 独立行政法人国立がん研究センター, 研究所, リサーチレジデント (30529459)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 頭蓋内胚細胞腫 / ジャーミノーマ / ゲノムワイドな低メチル化 / 始原生殖細胞由来 / メチル化 / 始原生殖細胞 |
|---|
| Outline of Final Research Achievements |
To elucidate the pathogenesis of intracranial germ cell tumors (iGCTs), we are going to execute a comprehensive genomic analysis by multi-institusional study. We used genomic DNA extracted from each frozen samples of 25 germinomas, 36 non-germinomatous GCTs (NGGCTs), and 6 normal tissues. A genome-wide methylation status was analyzed using HumanMethylation450 BeadChip (Illumina@). Germinomas were characterized by a global DNA hypomethylation in comparison to NGGCTs and normal tissues, which suggested germinomas originate from primordial germ cell showing a global hypomethylation during mammalian embryogenesis.
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