| Project/Area Number |
25830097
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Kanagawa Cancer Center Research Institute |
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Principal Investigator |
KANAYAMA Tomohiko 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), その他部局等, その他 (40401187)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | がん微小環境 / ハイポキシア / CD69 / インテグリン / 明細胞型卵巣癌 / 低酸素環境 |
|---|
| Outline of Final Research Achievements |
Ovarian clear cell carcinoma (CCC) is poor prognostic and frequent in Japan. We found that expression of CD69 gene is robustly and synergistically activated in CCC cells in response to serum starvation and hypoxia. CD69 is well known as a T cell activation marker, and is supposed to regulate the adequate immune response through its expressing on the surface of immune cells. However, the expression and characteristics of CD69 in solid tumor has never been characterized. To characterize the role of CD69 in CCCs, we performed the cell characteristic analyses under SSH condition. Then, CD69 dependent enhancement of cell migration and invasion activities with fibronectin as a chemoattractant were determined. In order to understand how CD69 facilitates association of CCC cells with FN, we performed various in vitro experiments. Our results suggest that CD69 enhances CCC cell-FN interaction through activation of cell surface integrin.
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