Development of novel diagnostic methods of serrated colorectal lesions

• Project/Area Number: 25830103
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Sapporo Medical University (2014); University of Miyazaki (2013)
• Principal Investigator: HARADA Taku 札幌医科大学, 医学部, 訪問研究員 (60468030)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 大腸がん / メチル化 / スクリーニング / 大腸鋸歯状病変 / 分子診断 / 腫瘍洗浄液 / 腸管洗浄液

Outline of Final Research Achievements

We assessed the clinical usefulness of aberrant DNA methylation in the bowel lavage fluid (BLF) for the colorectal tumor diagnosis. We found that a panel of three genes achieved highest sensitivities to detect colorectal cancer (miR-124-3, 71.8%; LOC386758, 79.5%; SFRP1, 74.4%). A scoring system based on the number of methylated genes (M-Score) enabled CRC detection with 82% sensitivity and 79% specificity.Our data indicate that DNA methylation in BLF could be a useful biomarker for colorectal cancer screening.

Research Products

• [Journal Article] Analysis of DNA methylation in bowel lavage fluid for detection of colorectal cancer. (2014)
  • Author(s): Harada T, Yamamoto E, Yamano HO, Nojima M, Maruyama R, Kumegawa K, Ashida M, Yoshikawa K, Kimura T, Harada E, Takagi R, Tanaka Y, Aoki H, Nishizono M, Nakaoka M, Tsuyada A, Niinuma T, Kai M, Shimoda K, Shinomura Y, Sugai T, Imai K, Suzuki H.
  • Journal Title: Cancer Prev Res (Phila) Volume: 7 Pages: 1002-10
  • Peer Reviewed
• [Journal Article] LSTの遺伝子学的特徴 遺伝子研究の立場からみたLSTの病態 (2014)
  • Author(s): 原田 拓、山本 英一郎、鈴木 拓、他
  • Journal Title: Intestine Volume: 8 Pages: 47-52
• [Journal Article] Aberrant methylation of microRNA-34b/c is a predictive marker of metachronous gastric cancer risk (2013)
  • Author(s): Suzuki R, Nosho K (他14名), Yamamoto H, Shinomura Y
  • Journal Title: J Gastroenterol Volume: (Epub ahead of print) Issue: 7 Pages: 1135-1144
  • DOI: 10.1007/s00535-013-0861-7
  • Peer Reviewed / Open Access
• [Presentation] DNA methylation in bowel lavage fluid is a useful biomarker for colorectal cancer screening. (2013)
  • Author(s): 原田 拓
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 腸管洗浄液のメチル化検出による大腸癌診断法の開発 (2013)
  • Author(s): 原田 拓
  • Organizer: 第33回日本分子腫瘍マーカー研究会
  • Place of Presentation: パシフィコ横浜