| Project/Area Number |
25830107
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnostics
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Kanayama Yousuke 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 研究員 (60435641)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 抗体PET / 64Cu / がんイメージング / キレーター / 抗体フラグメント / 非特異的集積 / 抗体PETイメージング / Cu-64 / フラグメント化抗体 / DOTA / NOTA / NODAGA / Zr-89 |
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| Outline of Final Research Achievements |
PET imaging of antibody drugs are expected to be highly useful for non-invasive diagnosis of target expression, selection of applicable patient, and treatment planning. To realize the antibody-based PET imaging, labeling method of positron emitting radiometal nuclide 64Cu has been investigated. In this study, we compared in vivo distribution of whole antibody, Fab, or F(ab’)2 fragments labeled with 64Cu using three different bifunctional chelator, DOTA-NHS-ester, SCN-Bn-NOTA, NODAGA-NHS-ester in the PET imaging of tumor-bearing mice.
As result, DOTA- or NODAGA-labeled Fab probes showed the increasing liver accumulation after the radioactivity in the heart (blood pool) was almost disappeared. The behavior of liver accumulation might be influenced by free 64Cu dissociated from chelators. On the contrary, NOTA-labeled probes showed lowest non-specific accumulations. Furthermore, it was indicated that linker structure was also able to affect the biodistribution.
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