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Comprehensive analysis of MHC-class I-restricted cancer antigen peptides

Research Project

Project/Area Number 25830108
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor diagnostics
Research InstitutionNational Cancer Center Japan

Principal Investigator

NARUMI Kenta  独立行政法人国立がん研究センター, その他部局等, 研究員 (10534258)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsペプチドワクチン / がん抗原 / 循環血中腫瘍細胞 / がんワクチン療法 / オーダーメイド治療
Outline of Final Research Achievements

Here we reported an acid-stripping-mediated comprehensive analysis method of MHC class I-restricted cancer antigen peptides. We added five HLA-A24-restricted Glypican3-related peptides peptides to the HLA-A24-expressing cells, following acid-stripping and ultrafiltration. The filtrates were analyzed by 2DICAL. It was revealed that only the peptide which had the strongest binding affinity against the HLA-A24 molecules was detected in the filtrates. This finding indicated that acid-stripping and 2DICAL analysis was a useful method to detect HLA-A24-binding peptides. This method may be applicable to the comprehensive screening of cancer antigen using circulating tumor cells.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (3 results)

  • [Journal Article] Suppression of Tregs by anti-GITR antibody enhances the antitumor immunity of IFN-alpha gene therapy for pancreatic cancer.2014

    • Author(s)
      K. Aida, R. Miyakawa, K. Suzuki, K. Narumi, T. Udagawa, Y. Yamamoto, T. Chikaraishi, T. Yoshida, K. Aoki.
    • Journal Title

      Cancer Science

      Volume: 105 Issue: 2 Pages: 636-645

    • DOI

      10.1111/cas.12332

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] VEGF-D-mediated blockade of regulatory T cells within tumors is induced by hematopoietic stem cell transplantation.2013

    • Author(s)
      T. Udagawa, K. Narumi, K.Suzuki, K. Aida, R. Miyakawa, Y. Ikarashi, A. Makimoto, T. Chikaraishi, T. Yoshida and K. Aoki.
    • Journal Title

      Journal of Immunology

      Volume: 191 Issue: 6 Pages: 3440-3452

    • DOI

      10.4049/jimmunol.1201454

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pre-immunization of donor lymphocytes enhances antitumor immunity of autologous hematopoietic stem cell transplantation.2013

    • Author(s)
      K. Suzuki, K. Aida, R. Miyakawa, K. Narumi, T. Udagawa, N. Goto, T. Yoshida, Y. Ohshima, K. Aoki.
    • Journal Title

      Cancer Medicine

      Volume: 2 Issue: 5 Pages: 636-645

    • DOI

      10.1002/cam4.117

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] S100A8/A9-mediated NK cell activation induces antitumor immunity after syngeneic hematopoietic stem cell transplantation2013

    • Author(s)
      Kenta Narumi, Reina Miyakawa, Ryosuke Ueda, Hisayoshi Hashimoto, Teruhiko Yoshida, Kazunori Aoki
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] A Cancer-targeting Ligand Strongly Enhances Oncolytic Activity of a Conditionally Replicative Adenovirus2013

    • Author(s)
      Yuki Yamamoto, Kazuki Miura, Naoko Goto, Kenta Narumi, Shumpei Ohnami, Teruhiko Yoshida, Masatoshi Tagawa, Kazunori Aoki
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] A Combination of IFN Gene Therapy and GITR Ab-induced Reduction of Tregs Enhances Tumor Immunity for Pancreatic Cancer2013

    • Author(s)
      Kazunori Aoki, Kouichirou Aida, Kenta Narumi, Reina Miyakawam Naoko Goto, Hisayoshi Hashimoto, Ryosuke Ueda, Yoshinori Ikarashi, Shumpei Ohnami, Teruhiko Yoshida
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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