Comprehensive analysis of MHC-class I-restricted cancer antigen peptides

• Project/Area Number: 25830108
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: National Cancer Center Japan
• Principal Investigator: NARUMI Kenta 独立行政法人国立がん研究センター, その他部局等, 研究員 (10534258)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ペプチドワクチン / がん抗原 / 循環血中腫瘍細胞 / がんワクチン療法 / オーダーメイド治療

Outline of Final Research Achievements

Here we reported an acid-stripping-mediated comprehensive analysis method of MHC class I-restricted cancer antigen peptides. We added five HLA-A24-restricted Glypican3-related peptides peptides to the HLA-A24-expressing cells, following acid-stripping and ultrafiltration. The filtrates were analyzed by 2DICAL. It was revealed that only the peptide which had the strongest binding affinity against the HLA-A24 molecules was detected in the filtrates. This finding indicated that acid-stripping and 2DICAL analysis was a useful method to detect HLA-A24-binding peptides. This method may be applicable to the comprehensive screening of cancer antigen using circulating tumor cells.

Research Products

• [Journal Article] Suppression of Tregs by anti-GITR antibody enhances the antitumor immunity of IFN-alpha gene therapy for pancreatic cancer. (2014)
  • Author(s): K. Aida, R. Miyakawa, K. Suzuki, K. Narumi, T. Udagawa, Y. Yamamoto, T. Chikaraishi, T. Yoshida, K. Aoki.
  • Journal Title: Cancer Science Volume: 105 Issue: 2 Pages: 636-645
  • DOI: 10.1111/cas.12332
  • Peer Reviewed
• [Journal Article] VEGF-D-mediated blockade of regulatory T cells within tumors is induced by hematopoietic stem cell transplantation. (2013)
  • Author(s): T. Udagawa, K. Narumi, K.Suzuki, K. Aida, R. Miyakawa, Y. Ikarashi, A. Makimoto, T. Chikaraishi, T. Yoshida and K. Aoki.
  • Journal Title: Journal of Immunology Volume: 191 Issue: 6 Pages: 3440-3452
  • DOI: 10.4049/jimmunol.1201454
  • Peer Reviewed
• [Journal Article] Pre-immunization of donor lymphocytes enhances antitumor immunity of autologous hematopoietic stem cell transplantation. (2013)
  • Author(s): K. Suzuki, K. Aida, R. Miyakawa, K. Narumi, T. Udagawa, N. Goto, T. Yoshida, Y. Ohshima, K. Aoki.
  • Journal Title: Cancer Medicine Volume: 2 Issue: 5 Pages: 636-645
  • DOI: 10.1002/cam4.117
  • Peer Reviewed
• [Presentation] S100A8/A9-mediated NK cell activation induces antitumor immunity after syngeneic hematopoietic stem cell transplantation (2013)
  • Author(s): Kenta Narumi, Reina Miyakawa, Ryosuke Ueda, Hisayoshi Hashimoto, Teruhiko Yoshida, Kazunori Aoki
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] A Cancer-targeting Ligand Strongly Enhances Oncolytic Activity of a Conditionally Replicative Adenovirus (2013)
  • Author(s): Yuki Yamamoto, Kazuki Miura, Naoko Goto, Kenta Narumi, Shumpei Ohnami, Teruhiko Yoshida, Masatoshi Tagawa, Kazunori Aoki
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] A Combination of IFN Gene Therapy and GITR Ab-induced Reduction of Tregs Enhances Tumor Immunity for Pancreatic Cancer (2013)
  • Author(s): Kazunori Aoki, Kouichirou Aida, Kenta Narumi, Reina Miyakawam Naoko Goto, Hisayoshi Hashimoto, Ryosuke Ueda, Yoshinori Ikarashi, Shumpei Ohnami, Teruhiko Yoshida
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜