The mechanisms of macrophage dependent vaccine

• Project/Area Number: 25830115
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics
• Research Institution: Mie University
• Principal Investigator: MURAOKA Daisuke 三重大学, 医学(系)研究科(研究院), 助教 (20608955)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: がんワクチン

Outline of Final Research Achievements

Previously we had identified that macrophage has critical role in the induction of immune responses elicited with CHP cancer vaccine (Macrophage oriented vaccine). In this study, to reveal the mechanisms of immune response induced with macrophage oriented vaccine, we compared with CHP cancer long peptide vaccine and the long peptide vaccine emulsified with IFA (classical dendritic cell dependent vaccine). As a result, we had demonstrated that the medullary macrophage has a critical role in macrophage oriented vaccine. Furthermore, we had shown the detailed mechanism which is involved in the efficacy of this vaccine system.

Research Products

• [Journal Article] Interleukin-17 Induces an Atypical M2-Like Macrophage Subpopulation That Regulates Intestinal Inflammation. (2014)
  • Author(s): Nishikawa K, Seo N, Torii M, Ma N, Muraoka D, Tawara I, Masuya M, Tanaka K, Takei Y, Shiku H, Katayama N, Kato T.
  • Journal Title: PLOS ONE Volume: 9 Issue: 9 Pages: e108494-e108494
  • DOI: 10.1371/journal.pone.0108494
  • Peer Reviewed / Open Access
• [Journal Article] A Nanogel-based Immunologically Stealth Vaccine Targets Macrophages in the Medulla of Lymph Node and Induces Potent Anti-Tumor Immunity (2014)
  • Author(s): D. Muraoka, N. Harada, T. Hayashi, Y. Tahara, F. Momose, S. Sawada, S. Mukai, K. Akiyoshi, H. Shiku,
  • Journal Title: ACS nano Volume: 8 Issue: 9 Pages: 9209-9218
  • DOI: 10.1021/nn502975r
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Systemic CD8+ T Cell-Mediated Tumoricidal Effects by Intratumoral Treatment of Oncolytic Herpes Simplex Virus with the Agonistic Monoclonal Antibody for Murine Glucocorticoid-Induced Tumor Necrosis Factor Receptor. (2014)
  • Author(s): Ishihara M, Seo N, Mitsui J, Muraoka D, Tanaka M, Mineno J, Ikeda H, Shiku H.
  • Journal Title: PLoS One Volume: 9 Issue: 8 Pages: e104669-e104669
  • DOI: 10.1371/journal.pone.0104669
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] CHPナノゲルはロングペプチド抗原をリンパ節内のマクロファージに抗原提示させる (2013)
  • Author(s): 村岡 大輔、原田 直純、林 妙、百瀬 文康、澤田 晋一、秋吉 一成、珠玖 洋
  • Organizer: 第17回 日本がん免疫学会総会
  • Place of Presentation: ANAクラウンプラザホテル宇部
• [Presentation] Peptide Sequence Design and Delivery System Are Critical for the Immunogenicity of Long Peptide-Based Cancer Vaccines (2013)
  • Author(s): Daisuke Muraoka, Naozumi Harada, Tae Hayashi, Kazunari Akiyoshi, and Hiroshi Shiku
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Macrophage is a good target as antigen presenting cell to induce strong immune responses elicted with administration of vaccination antigen and immunological adjuvant (2013)
  • Author(s): MURAOKA Daisuke, HARADA Naozumi, HAYASHI Tae, MOMOSE Fumiyasu, SAWADA Shin-ichi, AKIYOSHI Kazunari, SHIKU Hiroshi
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ