Investigating horizontal transferability of 16S ribosomal RNA gene
Project/Area Number |
25830132
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Genome biology
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Research Institution | Osaka University |
Principal Investigator |
KITAHARA Kei 大阪大学, 情報科学研究科, 研究員 (30567855)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 16S rRNA / メタゲノム / 大腸菌 / 16SリボソームRNA / 分子進化 / バクテリア / 遺伝子工学 / 進化能 / 遺伝子の水平伝播 |
Outline of Final Research Achievements |
In general, many prokaryotic genes are amenable to be transferred between different species. Hence, phylogeny of prokaryotic species has been reduced by comparing each species' 16S rRNA sequence. The rationale for this is based on an assumption that the 16S rRNA gene is least unlikely to be transferred between species. This assumption called "complexity hypothesis" was experimentally tested in this study, by introducing 16S rRNA genes from metagenome into Escherichia coli.
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Report
(3 results)
Research Products
(3 results)