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Identification and characterization of transposable element-regulating small RNAs

Research Project

Project/Area Number 25830134
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Genome biology
Research InstitutionKeio University

Principal Investigator

IWASAKI Yuka  慶應義塾大学, 医学部, 助教 (80612647)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords小分子RNA / トランスポゾン / 遺伝子発現抑制 / 生殖組織 / 非コードRNA / RNAサイレンシング / 転移因子 / 遺伝子発現制御 / Argonauteタンパク質
Outline of Final Research Achievements

Small RNAs mediate transposable element (TE) silencing by binding Argonaute/Piwi proteins. Here, we describe small RNA profiling of the adult testes of Callithrix jacchus, a model primate. We identified 353 novel miRNAs, which some originated from TEs. Meanwhile, the most abundant class of small RNAs in the adult testis was piRNAs. Therefore, we generated an antibody for MARWI, a marmoset PIWI protein, and performed high throughput sequencing analysis. MARWI-piRNAs show characteristics of mouse pachytene piRNAs, and are mostly derived from conserved clustered regions in the genome, known as pIRNA clusters. Further analysis revealed that strand bias observed for piRNAs mapped to each TE subfamily correlates with the polarity of TE copies inserted in clusters. Also, more piRNAs map to TE subfamilies when they have copies in piRNA clusters. These findings suggest that TE insertions to pachytene piRNA clusters determine abundance and strand-bias of TE-derived piRNAs.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2015 2014 2013 Other

All Journal Article (9 results) (of which Peer Reviewed: 7 results,  Open Access: 6 results,  Acknowledgement Compliant: 3 results) Presentation (4 results) (of which Invited: 2 results)

  • [Journal Article] Respective functions of two distinct Siwi complexes assembled during piRNA biogenesis in insect germ cells.2015

    • Author(s)
      Nishida, KM., Iwasaki, YW., Murota, Y., Nagao, A., Mannen, T., Kato, Y., Siomi, H., and Siomi, MC.
    • Journal Title

      Cell Reports

      Volume: 10 Issue: 2 Pages: 193-203

    • DOI

      10.1016/j.celrep.2014.12.013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] PIWI-interacting RNA: Its Biogenesis and Functions2015

    • Author(s)
      Iwasaki Y.W., Siomi M.C., Siomi H.
    • Journal Title

      Annual Review of Biochemistry

      Volume: 84 Issue: 1 Pages: 405-433

    • DOI

      10.1146/annurev-biochem-060614-034258

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate.2014

    • Author(s)
      Hirano T, Iwasaki YW, Lin ZY, Imamura M, Seki NM, Sasaki E, Saito K, Okano H, Siomi MC, *Siomi H.
    • Journal Title

      RNA

      Volume: 20 Issue: 8 Pages: 1223-1237

    • DOI

      10.1261/rna.045310.114

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Yb Integrates piRNA Intermediates and Processing Factors into Perinuclear Bodies to Enhance piRISC Assembly.2014

    • Author(s)
      Murota Y, Ishizu H, Nakagawa S, Iwasaki YW, Shibata S, Kamatani MK, Saito K, Okano H, Siomi H, Siomi MC.
    • Journal Title

      Cell Rep.

      Volume: 8 Issue: 1 Pages: 103-113

    • DOI

      10.1016/j.celrep.2014.05.043

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Epigenetic silencing of miR-210 increases the proliferation of gastric epithelium during chronic Helicobacter pylori infection2014

    • Author(s)
      Kiga K et.al
    • Journal Title

      Nat Commun

      Volume: 5 Issue: 1 Pages: 4497-4497

    • DOI

      10.1038/ncomms5497

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] miRNA regulatory ecosystem in early embryogenesis2014

    • Author(s)
      Iwasaki, YW and Siomi, H
    • Journal Title

      Molecular Cell

      Volume: 56 Issue: 5 Pages: 615-616

    • DOI

      10.1016/j.molcel.2014.11.010

    • Related Report
      2014 Annual Research Report
    • Open Access
  • [Journal Article] DmGTSF1 is necessary for Piwi–piRISC-mediated transcriptional transposon silencing in the Drosophila ovary2013

    • Author(s)
      Ohtani H et al.
    • Journal Title

      Genes Dev.

      Volume: 27 Issue: 15 Pages: 1693-1705

    • DOI

      10.1101/gad.221150.113

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Global microRNA elevation by inducible Exportin 5 regulates cell cycle entry2013

    • Author(s)
      Iwasaki, Y. W.; Kiga, K.; Kayo, H.; Fukuda-Yuzawa, Y.; Weise, J.; Inada, T.; Tomita, M.; Ishihama, Y..; Fukao, T.
    • Journal Title

      RNA

      Volume: 19 (4) Issue: 4 Pages: 490-7

    • DOI

      10.1261/rna.036608.112

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 小分子RNAを介したゲノムレベルの自己と非自己の認識2013

    • Author(s)
      岩崎由香、齋藤都暁
    • Journal Title

      実験医学

      Volume: 31 Pages: 98-106

    • Related Report
      2013 Research-status Report
  • [Presentation] Piwi-piRNA regulates association of linker histone H1 with target transposon loci in Drosophila2015

    • Author(s)
      Iwasaki Y.W., Ishizu H., Shibuya A., Iyoda Y., Siomi M.C., Siomi H., Saito K.
    • Organizer
      The Twentieth Annual Meeting of the RNA Society (RNA2015)
    • Place of Presentation
      Madison, Wisconsin, USA
    • Year and Date
      2015-05-26 – 2015-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Piwi-piRNA regulates association of histone H1 with target chromatin loci2014

    • Author(s)
      Iwasaki Y.W., Shibuya A., Ohtani H., Siomi M.C., Siomi H., Saito K.
    • Organizer
      Cell Symposia Regulatory RNAs
    • Place of Presentation
      Berkeley, California, USA
    • Year and Date
      2014-10-19 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 霊長類生殖組織におけるトランスポゾン抑制小分子RNAの解析2014

    • Author(s)
      岩崎由香、平野孝昌、Lin Zachary Yu-Ching, 今村公紀、關菜央美、佐々木えりか、齋藤都暁、岡野栄之、塩見美喜子、塩見春彦
    • Organizer
      日本遺伝学会第86回大会
    • Place of Presentation
      長浜バイオ大学、長浜
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 霊長類生殖組織における小分子RNAの統合的解析

    • Author(s)
      岩崎由香、平野孝昌、Zachary Yu-Ching Lin, 今村公紀、佐々木えりか、岡野栄之、齋藤都暁、塩見美喜子、塩見春彦
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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