Identification and characterization of transposable element-regulating small RNAs
Project/Area Number |
25830134
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Genome biology
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Research Institution | Keio University |
Principal Investigator |
IWASAKI Yuka 慶應義塾大学, 医学部, 助教 (80612647)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | 小分子RNA / トランスポゾン / 遺伝子発現抑制 / 生殖組織 / 非コードRNA / RNAサイレンシング / 転移因子 / 遺伝子発現制御 / Argonauteタンパク質 |
Outline of Final Research Achievements |
Small RNAs mediate transposable element (TE) silencing by binding Argonaute/Piwi proteins. Here, we describe small RNA profiling of the adult testes of Callithrix jacchus, a model primate. We identified 353 novel miRNAs, which some originated from TEs. Meanwhile, the most abundant class of small RNAs in the adult testis was piRNAs. Therefore, we generated an antibody for MARWI, a marmoset PIWI protein, and performed high throughput sequencing analysis. MARWI-piRNAs show characteristics of mouse pachytene piRNAs, and are mostly derived from conserved clustered regions in the genome, known as pIRNA clusters. Further analysis revealed that strand bias observed for piRNAs mapped to each TE subfamily correlates with the polarity of TE copies inserted in clusters. Also, more piRNAs map to TE subfamilies when they have copies in piRNA clusters. These findings suggest that TE insertions to pachytene piRNA clusters determine abundance and strand-bias of TE-derived piRNAs.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Respective functions of two distinct Siwi complexes assembled during piRNA biogenesis in insect germ cells.2015
Author(s)
Nishida, KM., Iwasaki, YW., Murota, Y., Nagao, A., Mannen, T., Kato, Y., Siomi, H., and Siomi, MC.
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Journal Title
Cell Reports
Volume: 10
Issue: 2
Pages: 193-203
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate.2014
Author(s)
Hirano T, Iwasaki YW, Lin ZY, Imamura M, Seki NM, Sasaki E, Saito K, Okano H, Siomi MC, *Siomi H.
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Journal Title
RNA
Volume: 20
Issue: 8
Pages: 1223-1237
DOI
Related Report
Peer Reviewed / Open Access
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