Development of a in vivo introduction method of large genes using the artificial chromosome vector
Project/Area Number |
25830137
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Medical genome science
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Research Institution | Tottori University |
Principal Investigator |
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Project Period (FY) |
2014-02-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 人工染色体ベクター / 濃縮 / 純化法 / 微小核細胞 |
Outline of Final Research Achievements |
Human artificial chromosome (HAC) vectors have the ability to delivery a huge gene since there is no size limitation for the DNA to be introduced into them. However, the transfer efficiency of HAC to the target cells is low. The transfer method is based on the microcell-mediated chromosome transfer. Therefore, the concentration of the microcells containing only HAC (HAC-MC) is effective to improve the transfer efficiency. In this study, we successfully established a novel method to concentrate HAC-MC. This method might be also useful for the development of a novel in vivo introduction method of HAC to embryos and individuals.
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Report
(4 results)
Research Products
(2 results)