| Project/Area Number |
25830141
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Medical genome science
|
|---|
| Research Institution | National Cancer Center Japan |
|---|
Principal Investigator |
MAMORU Kato 独立行政法人国立がん研究センター, 研究所, ユニット長 (40391916)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TATSUHIRO Shibata 独立行政法人国立がん研究センター, 研究所, 分野長 (90311414)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 一細胞シークエンス / 次世代シークエンサー / がんゲノム / がん細胞進化 / 発がん |
|---|
| Outline of Final Research Achievements |
It is important to reveal how cancer is generated and progressed since this leads to better cancer prevention and treatment. Tumor progression can be viewed as a Darwinian evolution, where only fitter cancer cells proliferate more. The simplest way to analyze this process is trace the genetic changes of single cells along time. We used a mouse model for human colon cancer and extracted the genomes of 30 single cells at each of the 3 time points along tumor progression. An algorithm to identify single cell's variants is necessary for analyzing single cell genomes and we developed such an algorithm, using single cell sequencing data.
|
