Identifying cancer cell evolution from early stages of cancer progression by single-cell sequencing
Project/Area Number |
25830141
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Medical genome science
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Research Institution | National Cancer Center Japan |
Principal Investigator |
MAMORU Kato 独立行政法人国立がん研究センター, 研究所, ユニット長 (40391916)
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Co-Investigator(Renkei-kenkyūsha) |
TATSUHIRO Shibata 独立行政法人国立がん研究センター, 研究所, 分野長 (90311414)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 一細胞シークエンス / 次世代シークエンサー / がんゲノム / がん細胞進化 / 発がん |
Outline of Final Research Achievements |
It is important to reveal how cancer is generated and progressed since this leads to better cancer prevention and treatment. Tumor progression can be viewed as a Darwinian evolution, where only fitter cancer cells proliferate more. The simplest way to analyze this process is trace the genetic changes of single cells along time. We used a mouse model for human colon cancer and extracted the genomes of 30 single cells at each of the 3 time points along tumor progression. An algorithm to identify single cell's variants is necessary for analyzing single cell genomes and we developed such an algorithm, using single cell sequencing data.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Development of Lung Adenocarcinomas with Exclusie Dependence on Oncogene Fusions.2015
Author(s)
Motonobu Saito, Yoko Shimada, Kouya Shiraishi, Hiromi Sakamoto, Koji Tsuta, Hirohiko Totsuka, Suenori Chiku, Hitoshi Ichikawa, Mamoru Kato, Shun-ichi Watanabe, Teruhiko Yoshida, Jun Yokota, Takashi Kohno
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Journal Title
Cancer Research
Volume: -
Issue: 11
Pages: 2264-2271
DOI
Related Report
Peer Reviewed
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[Journal Article] Profiling of actionable gene alterations in ovarian cancer by targeted deep sequencing.2015
Author(s)
Masataka Takenaka, Motonobu Saito, Reika Iwakawa, Nozomu Yanaihara, Misato Saito, Mamoru Kato, Hitoshi Ichikawa, Tatsuhiro Shibata, Jun Yokota, Aikou Okamoto, Takashi Kohno
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Journal Title
International Journal of Oncology
Volume: 46
Issue: 6
Pages: 2389-2398
DOI
Related Report
Peer Reviewed
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[Journal Article] Trans-ancestry mutational landscape of hepatocellular carcinoma genomes.2014
Author(s)
Yasushi Totoki, Kenji Tatsuno, Kyle R Covington, Hiroki Ueda, Chad J Creighton, Mamoru Kato, Shingo Tsuji, Lawrence A Donehower, Betty L Slagle, Hiromi Nakamura, Shogo Yamamoto, Eve Shinbrot, Natsuko Hama, Megan Lehmkuhl, Fumie Hosoda, Yasuhito Arai, Kim Walker, ・・・・ Hiroyuki Aburatani & Tatsuhiro Shibata
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Journal Title
Nature Genetics
Volume: 46
Pages: 1267-1273
Related Report
Peer Reviewed
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[Journal Article] Molecular mechanisms underlying oncogenic RET fusion in lung adenocarcinoma.2014
Author(s)
Tatsuji Mizukami, Kouya Shiraishi, Yoko Shimada, Hideaki Ogiwara, Koji Tsuta, Hitoshi Ichikawa, Hiromi Sakamoto, Mamoru Kato, Tatsuhiro Shibata, Takashi Nakano, Takashi Kohno,
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Journal Title
Journal of Thoracic Oncology
Volume: 9
Pages: 622-630
Related Report
Peer Reviewed
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[Journal Article] Mutually exclusive mutations of KIT and RAS are associated with KIT mRNA expression and chromosomal instability in primary intracranial pure germinomas2014
Author(s)
Shintaro Fukushima, Ayaka Otsuka, Tomonari Suzuki, Takaaki Yanagisawa, Kazuhiko Mishima, Akitake Mukasa, Nobuhito Saito, Toshihiro Kumabe, ・・・・Mamoru Kato, Tatsuhiro Shibata, ・・・・ Koichi Ichimura, On behalf of the Intracranial Germ Cell Tumor Genome Analysis Consortium (iGCT Consortium)
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Journal Title
Acta Neuropathologica
Volume: 127
Pages: 911-925
Related Report
Peer Reviewed
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[Journal Article] NCYM, a de novo evolved protein, stabilizes MYCN and characterizes human neuroblastoma.2014
Author(s)
Yusuke Suenaga, S. M. Rafiqul Islam, Jennifer Alagu, Yoshiki Kaneko, Mamoru Kato, Yukichi Tanaka, Hidetada Kawana, Shamim Hossain, Daisuke Matsumoto, Mami Yamamoto, Wataru Shoji, Makiko Itami, Tatsuhiro Shibata, Yohko Nakamura, Miki Ohira, Seiki Haraguchi, Atsushi Takatori, Akira Nakagawara.
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Journal Title
Related Report
Peer Reviewed
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