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Visualization of the whole metabolic network through absolute protein quantification

Research Project

Project/Area Number 25830143
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field System genome science
Research InstitutionKyushu University

Principal Investigator

MATSUZAKI Fumiko  九州大学, 生体防御医学研究所, 助教 (10631773)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords定量プロテオミクス / 代謝ネットワーク / 絶対量ベース代謝システム解析
Outline of Final Research Achievements

In this project, I focused on an absolute quantification of metabolic enzymes in each mouse organ and analyzing metabolic network for a comprehensive understanding of the metabolic system in individual level. I optimized the method for absolute protein quantification with our information-based quantitative proteomics technique and developed a novel mathematical method “Absolute quantity-based metabolic systems analysis (AMSA)”, which provides integrated analysis of metabolic network in each mouse organ.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (15 results)

All 2015 2014 2013 Other

All Presentation (11 results) (of which Invited: 4 results) Book (2 results) Remarks (2 results)

  • [Presentation] Absolute quantification of all human metabolic enzymes and metabolic systems analysis2015

    • Author(s)
      Fumiko Matsuzaki, Masaki Matsumoto, Kiyotaka Oshikawa, Keiichi Nakayama
    • Organizer
      First symposium: From genes to human diseases
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Absolute quantification of all human metabolic enzymes and metabolic systems analysis2015

    • Author(s)
      Fumiko Matsuzaki, Masaki Matsumoto, Kiyotaka Oshikawa, Keiichi Nakayama
    • Organizer
      The Third BMIRC International Symposium for Virtual Physiological Human
    • Place of Presentation
      Iizuka, Japan
    • Year and Date
      2015-03-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒト全代謝酵素の絶対定量と代謝システム解析2015

    • Author(s)
      松﨑 芙美子, 松本 雅記, 押川 清孝, 中山 敬一
    • Organizer
      定量生物学の会 第七回年会
    • Place of Presentation
      福岡
    • Year and Date
      2015-01-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Absolute quantification of all human metabolic enzymes and metabolic systems analysis2014

    • Author(s)
      Fumiko Matsuzaki, Masaki Matsumoto, Kiyotaka Oshikawa, Keiichi Nakayama
    • Organizer
      The 37th annual meeting of the molecular biology society of Japan
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 次世代ターゲットプロテオミクスによるヒト全代謝経路の絶対定量マッピング2014

    • Author(s)
      松本 雅記, 押川 清孝, 松﨑 芙美子, 五島 直樹, 夏目 徹, 中山 敬一
    • Organizer
      第87回生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ヒト全代謝酵素の絶対定量によるがん代謝ネットワークの統合解析2014

    • Author(s)
      松﨑 芙美子, 松本 雅記, 押川 清孝, 中山 敬一
    • Organizer
      第87回生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Absolute quantification of all metabolic enzymes by information-based proteomics and computational analysis for cancer metabolism2014

    • Author(s)
      Fumiko Matsuzaki
    • Organizer
      Seminar at Kyushu University
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2014-09-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ヒト全代謝酵素の絶対定量とグローバル代謝の数理解析2014

    • Author(s)
      松﨑 芙美子, 松本 雅記, 押川 清孝, 中山 敬一
    • Organizer
      第2回数理デザイン道場
    • Place of Presentation
      伊豆
    • Year and Date
      2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] タンパク質相互作用ネットワークにおける相互作用強度特性の探索2013

    • Author(s)
      松崎芙美子、松本雅記、押川清孝、高見知代、*中山敬一
    • Organizer
      第10回ネットワーク生態学シンポジウム
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] Absolute quantification of all metabolic enzymes by information-based proteomics and computational analysis for cancer metabolism2013

    • Author(s)
      Matsuzaki F., Matsumoto M., Oshikawa K., *Nakayama K.I.
    • Organizer
      Symposium on complex biodynamics and networks
    • Place of Presentation
      Yamagata, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒト全代謝酵素の絶対定量とグローバル代謝の数理解析2013

    • Author(s)
      松崎芙美子、松本雅記、押川清孝、*中山敬一
    • Organizer
      定量生物学の会 第六回年会
    • Place of Presentation
      大阪
    • Related Report
      2013 Research-status Report
  • [Book] がん分子標的治療2014

    • Author(s)
      松崎芙美子、松本雅記、中山敬一
    • Publisher
      メディカルレビュー社
    • Related Report
      2013 Research-status Report
  • [Book] 細胞工学2013

    • Author(s)
      松崎芙美子
    • Total Pages
      1
    • Publisher
      秀潤社
    • Related Report
      2013 Research-status Report
  • [Remarks] 九州大学生体防御医学研究所統合オミクス分野

    • URL

      http://www.bioreg.kyushu-u.ac.jp/labo/omics/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 九州大学生体防御医学研究所分子医科学分野

    • URL

      http://www.bioreg.kyushu-u.ac.jp/saibou/index.html

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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