Visualization of the whole metabolic network through absolute protein quantification
Project/Area Number |
25830143
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
System genome science
|
Research Institution | Kyushu University |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 定量プロテオミクス / 代謝ネットワーク / 絶対量ベース代謝システム解析 |
Outline of Final Research Achievements |
In this project, I focused on an absolute quantification of metabolic enzymes in each mouse organ and analyzing metabolic network for a comprehensive understanding of the metabolic system in individual level. I optimized the method for absolute protein quantification with our information-based quantitative proteomics technique and developed a novel mathematical method “Absolute quantity-based metabolic systems analysis (AMSA)”, which provides integrated analysis of metabolic network in each mouse organ.
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Report
(3 results)
Research Products
(15 results)