| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
By introducing mutations on the phosphorylation sites of CRY1, one of the core component of mammalian circadian clock, many of these residues were found to be important for proper circadian period and/or robust amplitude both in cultured cell and mice. Analysis of the mutation effect on CRY1 properties re-emphasizes close coupling between CRY1 turnover rate and the circadian period. This relationship are rigorously tested by applying small-molecule-inducible degradation of CRY1.
Nevertheless, several mutants show markedly altered circadian period but less alter the CRY1 stability. These mutations are located mostly around the FAD-binding pocket of cryptochrome. We further confirmed that residues not directly linked to phosphorylation/electron-transferring but supposed to be retain the structure of the two domain also alter the period.
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