Intracellular self-organization of actin cytoskeleton

• Project/Area Number: 25830147
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: FUJITA Masashi 独立行政法人理化学研究所, 生命システム研究センター, 研究員 (80564749)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 細胞骨格 / アクチン / ミオシン / 細胞動態 / RhoGAP / アクチン細胞骨格 / 細胞内局在 / Rho / 線虫

Outline of Final Research Achievements

Actin filaments and myosin motor form network at the cell cortex and generate contractile forces. This actomyosin network form pulsatile foci in the cell cortex of the one-cell embryos of the nematode C. elegans. In this study, I investigated how the foci structure is regulated. I found that RhoGAP proteins RGA-3/4 colocalize to the actomyosin foci. Experiments showed that RGA-3/4 regulate the size and disassembly of actomyosin foci.

Research Products

• [Presentation] Colocalization of RhoGAP Restricts the Size of Pulsatile Actomyosin Foci in C. elegans Embryos (2014)
  • Author(s): Fujita M., Onami S.
  • Organizer: The 2014 ASCB/IFCB Meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2014-12-09
• [Presentation] Colocalization of RhoGAP Restricts the Size of Pulsatile Actomyosin Foci in C. elegans Embryos (2014)
  • Author(s): Fujita M., Onami S.
  • Organizer: The 62nd NIBB Confrnce "Force in Development"
  • Place of Presentation: 岡崎コンファレンスセンター（岡崎市）
  • Year and Date: 2014-11-17
• [Presentation] Morphology of actomyosin network is regulated by colocalization of RhoGAP RGA-3/4 (2014)
  • Author(s): Fujita M., Onami S.
  • Organizer: C. elegans Development, Cell Biology and Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting
  • Place of Presentation: 奈良県新公会堂（奈良市）
  • Year and Date: 2014-07-16
• [Presentation] RhoGAP proteins RGA-3/4 mediate spatial negative feedback of the actomyosin in C. elegans embryos (2013)
  • Author(s): 藤田征志, 大浪修一
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都
• [Presentation] Analysis of colocalization between RhoGAP protein RGA-3/4 and actomyosin in C. elegans embryos (2013)
  • Author(s): 藤田征志, 大浪修一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸