Elucidation of the piRNA pathway and development of novel gene knock-down method
| Project/Area Number |
25840004
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
ISHIZU Hirotsugu 東京大学, 理学(系)研究科(研究院), 助教 (40574588)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Keywords | piRNA / Piwi / RNAサイレンシング / RNA silencing |
|---|
| Outline of Final Research Achievements |
PIWI proteins, germline-specific members of the Argonaute family, function in RNA silencing in the gonad by associating specifically with PIWI-interacting RNAs (piRNAs). We found that it is possible to generate piRNAs that were artificially designed, using drosophila ovarian somatic cells. In this research, we developed the novel gene knockdown method using artificial piRNAs. We constructed the piRNA expression vectors that produces artificial piRNAs targeting optional genes, and examined the silencing effect by artificial piRNAs. As a result, artificial piRNAs succeeded in silencing of the endogenous gene, and we found that this suppression was occured at the transcriptional level by piRNA pathway.
|
Report
(3 results)
Research Products
(5 results)
