| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
In this study, I attempted to reveal how the nuclear envelope regulates transcription thorough analyzing the gene regulation mechanism by one of the nuclear membrane proteins, lamin B receptor (LBR), at genome-wide level. I investigated the relationship between the LBR-binding and the transcriptional level of the gene across the whole genome before and after differentiation of HL60 cells by retinoic acid treatment, and found that LBR regulated extracellular matrix-related genes and its pathway. Those genes were also varied in LBR-knocked down HeLa cells. Taken together, those results suggested that the nuclear envelope plays a role to create an extracelluar environment in response to a biological signal such as differentiation.
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