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Regulatory mechanism controlling a novel nuclease activity of Mre11 complex on structured DNA

Research Project

Project/Area Number 25840009
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

FURUKOHRI Asako  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (90546293)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsDNA修復 / Mre11 complex / DNA二本鎖切断 / Mrer11 complex
Outline of Final Research Achievements

The Mre11/Rad50 complex has important roles in various genome maintenance pathways. In this research project, we analyzed a novel type of nuclease activity found for Escherichia coli Mre11/Rad50 complex, SbcCD. Unexpectedly, nuclease activities of SbcCD were not significantly affected by a secondary structure on substrate DNA, but we found that the activity of SbcCD is modulated by the presence of DNA end. SbcCD specifically cuts off the top of a cruciform DNA or the end of dsDNA by making incisions on both strands. The finding of this a novel, DNA-end dependent nuclease activity will provide new insights in the future research and may help us understand the role of the Mre11/Rad50 complex in the human cell.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014

All Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 4 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Long inverted repeat transiently stalls DNA replication by forming hairpin structures on both leading and lagging strands2016

    • Author(s)
      Lai P.J., Lim C.T., Le H.P., Katayama T., Leach D.R.F, Furukohri A*., Maki H.
    • Journal Title

      Genes to Cells

      Volume: 21 Issue: 2 Pages: 136-145

    • DOI

      10.1111/gtc.12326

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A novel mode of nuclease action is revealed by the bacterial Mre11/Rad50 complex2015

    • Author(s)
      Lim C.T., Lai P.J., Leach D.R.F, Maki H., Furukohri A
    • Journal Title

      Nucleic Acids Research

      Volume: 43 Pages: 9804-9816

    • DOI

      10.1093/nar/gkv855

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Short CCG repeat in huntingtin gene is an obstacle for replicative DNA polymerases, potentially hampering progression of replication fork.2015

    • Author(s)
      Le HP, Masuda Y, Tsurimoto T, Maki S, Katayama T, Furukohri A, Maki H.
    • Journal Title

      Genes Cells.

      Volume: 10 Issue: 10 Pages: 817-833

    • DOI

      10.1111/gtc.12275

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Recombinase and translesion DNA polymerase decrease the speed of replication fork progression during the DNA damage response in Escherichia coli cells.2015

    • Author(s)
      Tan K.W., Pham M.T., Furukohri A, Maki H. and Akiyama T.M.
    • Journal Title

      Nucleic Acids Research

      Volume: 43 Issue: 3 Pages: 1715-1725

    • DOI

      10.1093/nar/gkv044

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] DNA polymeraes IV mediates efficient and quick recovery at N2-dG adducts.2014

    • Author(s)
      Ikeda M, Furukohri A, Philippin G, Loechler E, Akiyama MT, Katayama T, Fuchs RP, Maki H.
    • Journal Title

      Nucleic Acids Research

      Volume: 42 Issue: 13 Pages: 8461-8472

    • DOI

      10.1093/nar/gku547

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 大腸菌Mre11/Rad50ホモログSbcCDの新規DNA末端依存的二本鎖DNA切断活性の生化学的解析2015

    • Author(s)
      古郡 麻子、Lim Chew Theng、David R.F. Leach、真木 寿治
    • Organizer
      第23回DNA複製・組換え・修復ワークショップ
    • Place of Presentation
      焼津グランドホテル(静岡県焼津市)
    • Year and Date
      2015-10-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Biochemical analysis reveals a novel action of bacterial Mre11/Rad50 complex2015

    • Author(s)
      古郡 麻子、Lim Chew Theng、David R.F. Leach、真木 寿治
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      東北大学(宮城県仙台市)
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 大腸菌損傷乗り越え型DNA Polymerase IVの複製フォークにおける役割2015

    • Author(s)
      古郡 麻子、池田 美央、秋山 昌広、片山 勉、Robert P. Fuchs、真木 寿治
    • Organizer
      第12回21世紀大腸菌研究会
    • Place of Presentation
      琵琶湖グランドホテル(滋賀県大津市)
    • Year and Date
      2015-06-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] Escherichia coli DNA polymerase IV mediates efficient and quick recovery of replication forks stalled at N2-dG adducts2014

    • Author(s)
      Asako Furukohri, Mio Ikeda, Masahiro Tatsumi Akiyama, Tsutomu Katayama, Robert P. Fuchs and Hisaji Maki
    • Organizer
      The 9th 3R Symposium
    • Place of Presentation
      御殿場高原ホテル、御殿場市
    • Year and Date
      2014-11-17 – 2014-11-21
    • Related Report
      2014 Research-status Report
  • [Presentation] Escherichia coli DNA polymerase IV mediates efficient and quick recovery of replication forks stalled at N2-dG adducts2014

    • Author(s)
      Asako Furukohri, Mio Ikeda, Masahiro Tatsumi Akiyama, Tsutomu Katayama, Robert P. Fuchs and Hisaji Maki
    • Organizer
      DNA polymerases: Biology, Diseases and Biomedical Applications Conference 2014
    • Place of Presentation
      Robinson College, Cambridge, England, UK
    • Year and Date
      2014-08-31 – 2014-09-04
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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