Analysis of piRNA biosynthesis mechanism and identification of its target gene

Research Project

Project/Area Number	25840010
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Molecular biology
Research Institution	Nagasaki University
Principal Investigator	NAKAGAWA Takeya 長崎大学, 医歯薬学総合研究科(医学系), 助教 (50363502)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	piRNA / 試験管内遺伝子転写
Outline of Final Research Achievements	In the present study, we investigated mechanism of piRNA biosynthesis and its target gene. To elucidate the mechanism of piRNA transcription, we established the in vitro transcription system which can transcribes piRNA from whole genome DNA. This system is useful to identify the factor which regulates piRNA transcription.　Furthermore, this system can analyze target gene of piRNA comprehensively.