| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The dynamic oligomerized proteins, Coiled-coil DIX1(Ccd1) and Axin, play an important role in the regulation of the Wnt signaling pathway by forming homotypic and heterotypic oligomers between the DIX domains. The structural study for the Ccd1-Axin hetero-oligomer using X-ray crystallography reveals the heterotypic interaction of the Ccd1 DIX domain with the Axin DIX domain and a significance of the hetero-oligomerization in the regulation of the Wnt signaling pathway. This report describes the preparation, crystallization, X-ray diffraction and structural analysis of the Ccd1-Axin hetero-oligomer. The crystals of the Ccd1-Axin hetero-oligomer diffracted to a resolution of 3.1 angstrom. Structural analysis of the Ccd1-Axin hetero-oligomer is now in progress.
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