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Structural and functional analyses of Mmm1 and Mdm12 in the ERMES complex

Research Project

Project/Area Number 25840020
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionKyoto Sangyo University (2014-2015)
Nagoya University (2013)

Principal Investigator

KAWANO Shin  京都産業大学, 総合生命科学部, 研究助教 (90431676)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords結晶構造 / オルガネラネットワーク / リン脂質 / タンパク質複合体 / リン脂質輸送 / ホモロジーモデル / 単離ミトコンドリア / 結晶構造解析 / リポソーム
Outline of Final Research Achievements

The ERMES complex was recently found to be involved in the tethering system between ER and mitochondria in yeast. The complex consists of four core components (Mmm1, Mmm2, Mdm10, and Mdm12) and is supposed as a transducer of phospholipids between those two organelle. In this study, we succeeded in determining the crystal structure of Mdm12. In addition, the functional analyses of Mdm12 and Mdm12-Mmm1 soluble domain complex were also performed.
The crystal structure of Mdm12 belongs to SMP domain fold, which can accommodate a phospholipid in its cave structure. Furthermore, in vitro lipid transfer assay using Mdm12 and Mdm12-Mmm1 complex showed that the lipid transfer activity of Mdm12 was largely enhanced by forming a hetero oligomeric complex with Mmm1. These observations indicate that ERMES complex transfers phospholipids between the ER and mitochondrial outer membrane via Mdm12-Mmm1 complex as a minimal unit.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (9 results)

All 2015 2014 2013

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Invited: 1 results)

  • [Journal Article] Structural and mechanistic insights into phospholipid transfer by Ups1–Mdm35 in mitochondria.2015

    • Author(s)
      Watanabe, Y., Tamura, Y., Kawano, S., and Endo T.
    • Journal Title

      Nature Communications

      Volume: 6 Issue: 1 Pages: 772-772

    • DOI

      10.1038/ncomms8922

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Mdm12とMmm1によるリン脂質輸送メカニズムの解明2015

    • Author(s)
      河野慎、遠藤斗志也
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膜間リン脂質輸送の構造生物学2015

    • Author(s)
      河野慎、渡邊康紀、田村康、遠藤斗志也
    • Organizer
      第67回細胞生物学会大会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ERMES複合体によるリン脂質輸送の構造基盤2015

    • Author(s)
      河野慎、遠藤斗志也
    • Organizer
      第15回蛋白質科学会年会
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] ERMES複合体構成因子Mdm12およびMmm1によるリン脂質輸送メカニズムの解明2014

    • Author(s)
      河野慎、浅井絵里、スチンバラ、遠藤斗志也
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都市左京区岩倉大鷺町422番地国立京都国際会館
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 小胞体ーミトコンドリア繋留複合体(ERMES複合体)構成因子Mdm12の構造機能相関2014

    • Author(s)
      河野慎、浅井絵里、スチンバラ、遠藤斗志也
    • Organizer
      第14回日本蛋白質科学会年会
    • Place of Presentation
      神奈川県横浜市中区山下町24-1ワークピア横浜
    • Year and Date
      2014-06-25 – 2014-06-27
    • Related Report
      2014 Research-status Report
  • [Presentation] ERMES複合体構成タンパク質Mdm12の構造解析2013

    • Author(s)
      河野慎,スチンバラ,遠藤斗志也
    • Organizer
      第13回日本蛋白質科学会年会
    • Place of Presentation
      鳥取
    • Related Report
      2013 Research-status Report
  • [Presentation] ERMES複合体構成タンパク質Mdm12の構造解析2013

    • Author(s)
      河野慎,スチンバラ,遠藤斗志也
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] Structural analysis of Mdm122013

    • Author(s)
      Shin Kawano, Si-quinbala, Eri Asai, and Toshiya Endo
    • Organizer
      DynaMito 2013: The 4th International Symposium on Dynamics of Mitochondria - from Molecular Mechanisms to Physiological Functions and Disease
    • Place of Presentation
      沖縄
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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