Structure and function of moyamoya disease-associated protein, mysterin
Project/Area Number |
25840024
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | Kyoto Sangyo University |
Principal Investigator |
MORITO Daisuke 京都産業大学, 総合生命科学部, 研究員 (20514251)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | ミステリン / モヤモヤ病 / AAA+タンパク質 / ユビキチン / mysterin / RNF213 / AAA+ ATPアーゼ |
Outline of Final Research Achievements |
We demonstrated that mysterin contains enzymatically active AAA+ ATPase modules, forms large troidal oligomer through these modules, and dynamically changes its structural state. Furthermore, we demonstrated that mysterin is indispensble for neuro, muscular, and vascular development in vivo using zebrafish model. The two enzymatic activities of mysterin are both essential for this function.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] The moyamoya disease susceptibility variant RNF213 R4810K (rs112735431)2013
Author(s)
Hitomi T, Habu T, Kobayashi H, Okuda H, Harada KH, Osafune K, Taura D, Sone M, Asaka I, Ameku T, Watanabe A, Kasahara T, Sudo T, Shiota F, Hashikata H, Takagi Y, Morito D, Miyamoto S, Nakao K, Koizumi A
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Journal Title
Biochem Biophys Res Commun.
Volume: 439(4)
Issue: 4
Pages: 419-426
DOI
Related Report
Peer Reviewed
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[Journal Article] Downregulation of Securin by the variant RNF213 R4810K (rs112735431, G>A) reduces angiogenic activity of induced pluripotent stem cell-derived vascular endothelial cells from moyamoya patients.2013
Author(s)
Hitomi T, Habu T, Kobayashi H, Okuda H, Harada KH, Osafune K, Taura D, Sone M, Asaka I, Ameku T, Watanabe A, Kasahara T, Sudo T, Shiota F, Hashikata H, Takagi Y, Morito D, Miyamoto S, Nakao K, Koizumi A.
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Journal Title
Biochem Biophys Res Commun.
Volume: 438(1):
Issue: 1
Pages: 13-19
DOI
Related Report
Peer Reviewed
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[Presentation] Structure and function of moyamoya disease-associated AAA+ ATPase/ubiquitin ligase mysterin2013
Author(s)
Morito D, Kotani Y, Nishikawa K, Hoseki J, Yamazaki S, Iemura S, Natsume T, Takashima S, Fujiyoshi Y, Nagata K
Organizer
The 35th Naito Conference: The Ubiquitin-Proteasome System
Place of Presentation
Chateux Gateaux Kingdom Sapporo, Sapporo, Japan
Related Report
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