High-energy conformations of proteins as a new target of structural biology
| Project/Area Number |
25840025
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
Ryo Kitahara 立命館大学, 薬学部, 准教授 (70512284)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 圧力 / コンフォメーション / NMR / 熱力学 / 蛍光 / 変性中間体 / OspA / TDP43 / pressure / high-energy state / TDP-43 / RRM |
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| Outline of Final Research Achievements |
The conventional NMR approach for structure determination so far reveals atomic coordinates of the most stable conformation of a protein, the so-called “native state”. Despite significant progress in our knowledge about the basic folded conformation of proteins, high-Gibbs free energy states of proteins are poorly understood. Here, we investigate the structure and backbone dynamics of locally disordered state of ubiquitin using high pressure NMR spectroscopy. We carried out NMR signal assignments, the collection of structural information based on chemical shifts, NOE, and paramagnetic relaxation enhancements (PRE). We also performed 15N spin relaxation experiments (T1, T2, hNOE). More than 1000 NOE- and PRE-based distance constraints and 42 torsion angle constraints were used for structure calculation of the locally disordered state. High-pressure NMR spectroscopy provides snapshots of fluctuating ubiquitin structure at atomic resolution.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Aberrant assembly of RNA-recognition motif 1 links to pathogenic conversion of TAR DNA-binding protein-43 (TDP-43)2013
Author(s)
Shodai A, Morimura T, Ido A, Uchida T, Ayaki T, Takahashi R, Kitazawa S, Suzuki S, Shirouzu M, Kigawa T, Muto Y, Yokoyama S, Takahashi R, Kitahara R, Ito H, Fujiwara N, Urushitani M
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Journal Title
J Biol Chem
Volume: 288(21)
Issue: 21
Pages: 14886-14905
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] NMR snapshots of a fluctuating ubiquitin structure2016
Author(s)
Soichiro Kitazawa, Takuro Wakamoto, Teppei Ikeya, Tomoshi Kameda, Maho Yagi-Utsumi, Koichi Kato, Christian Roumestand, Nicola J. Baxter, Mike P. Williamson, Ryo Kitahara
Organizer
The annual international EUROMAR conference 2016
Place of Presentation
オーフスコンサートホール（デンマーク）
Year and Date
2016-07-06
Related Report
Int'l Joint Research / Invited
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