Exploration of Golgi casein kinases

• Project/Area Number: 25840031
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Chiba University
• Principal Investigator: Ishikawa Hiroyuki 千葉大学, 理学(系)研究科(研究院), 准教授 (00398819)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ゴルジ体キナーゼ / リン酸化 / 翻訳後修飾 / 分泌経路

Outline of Final Research Achievements

Casein is a secretory phosphorylated protein containing in milk. We attempted to identify the kinase molecule to phosphorylate casein in the Golgi. As a result, we identified candidate genes for novel Golgi kinases in the Drosophila genome. We also succeeded in producing Drosophila mutants of these genes. Further, we found that overexpression of a Golgi kinase FAM20C affected the normal development of Drosophila.

Research Products

• [Journal Article] O-fucose monosaccharide of Drosophila Notch has a temperature-sensitive function and cooperates with O-glucose glycan in Notch transport and Notch signaling activation (2015)
  • Author(s): Akira Ishio, Takeshi Sasamura, Tomonori Ayukawa, Junpei Kuroda, Hiroyuki O. Ishikawa, Naoki Aoyama, Kenjiroo Matsumoto, Takuma Gushiken, Tetsuya Okajima, Tomoko Yamakawa and Kenji Matsuno2
  • Journal Title: Jounal of Biochemistry Volume: 290 Issue: 1 Pages: 505-519
  • DOI: 10.1074/jbc.m114.616847
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A test of double interspecific introgression of nucleoporin genes in Drosophila (2014)
  • Author(s): K.Sawamura, K.Maehara, Y.Keira, H.O.Ishikawa, T.Sasamura. T.Yamakawa, K.Matsuno
  • Journal Title: G3 Genes, Genomes, Genetics Volume: 4 Issue: 11 Pages: 2101-2106
  • DOI: 10.1534/g3.114.014027
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A gain-of-function screen to identify genes that reduce lifespan in the adult of Drosophila melanogaster. (2014)
  • Author(s): Nakayama M, Ishibashi T, Ishikawa HO, Sato H, Usui T, Okuda T, Yashiro H, Ishikawa H, Taikou Y, Minami A, Kato K, Taki M, Aigaki T, Gunji W, Ohtsu M, Murakami Y, Tanuma SI, Tsuboi A, Adachi M, Kuroda J, Sasamura T, Yamakawa T, Matsuno K.
  • Journal Title: BMC Genet Volume: 15 Pages: 46-46
  • Peer Reviewed
• [Presentation] ゴルジ体キナーゼFour-jointedの発現・局在パターン形成機構 (2015)
  • Author(s): 松本仙太郎、白石穂高、計良陽子、石川裕之
  • Organizer: BMB2015（第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートアイランド（兵庫県・神戸市）
  • Year and Date: 2015-12-03
  • Invited
• [Presentation] ショウジョウバエの強制発現系を用いた平面細胞極性を制御する遺伝子の探索 (2015)
  • Author(s): 田中友子、和田萌、計良陽子、石川裕之
  • Organizer: 日本動物学会第67回関東支部大会
  • Place of Presentation: 早稲田大学
  • Year and Date: 2015-03-14
• [Presentation] Subcellular localization of the Golgi kinase Four-jointed (2015)
  • Author(s): Hodaka Shiraishi, Hirofumi Nonoyama, Yoko Keira, Hiroyuki O. Ishikawa
  • Organizer: 56th Annual Drosophila Research Conference
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-03-05 – 2015-03-07
• [Presentation] ショウジョウバエ成虫原基におけるfour-jointedのエンハンサー解析 (2014)
  • Author(s): 松本仙太郎、荒井星矢、計良陽子、石川裕之
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-27
• [Presentation] ゴルジ体キナーゼFour-jointedの細胞内局在化機構の解析 (2014)
  • Author(s): 白石穂高、野々山裕文、佐々木飛鳥、土井由香、野上識、計良陽子、石川裕之
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-27
• [Presentation] Subcellular localization and domain analysis of the Golgi kinase Four-jointed (2014)
  • Author(s): Hodaka Shiraishi, Yoko Keira and Hiroyuki O. Ishikawa
  • Organizer: The 62nd NIBB Conference, Force in Development
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2014-11-18
• [Presentation] Identification of enhancers of four-jointed in Drosophila imaginal discs (2014)
  • Author(s): Sentaro Matsumoto, Seiya Arai, Yoko Keira, Hiroyuki O. Ishikawa
  • Organizer: 11th Japanese Drosophila Research Conference
  • Place of Presentation: 金沢歌劇座
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] Subcellular localization and domain analysis of the Golgi kinase Four-jointed (2014)
  • Author(s): Hodaka Shiraishi, Hirofumi Nonoyama, Asuka Sasaki, Yoko Keira, Hiroyuki O. Ishikawa
  • Organizer: 11th Japanese Drosophila Research Conference
  • Place of Presentation: 金沢歌劇座
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] タンパク質細胞外領域のリン酸化：古くて新しい翻訳後修飾
  • Author(s): 石川裕之、野々山裕文、田中友子、計良陽子
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
  • Invited
• [Presentation] ゴルジ体キナーゼFour-jointedの細胞内局在制御機構の解析
  • Author(s): 野々山裕文、中山綾子、佐々木飛鳥、計良陽子、石川裕之
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Presentation] ゴルジ体キナーゼFour-jointedの自己リン酸化の機能解析
  • Author(s): 田中友子、中山綾子、石川裕之
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
• [Remarks] 千葉大学 大学院理学研究科 生物学教室 発生遺伝学研究室
  • URL: http://life.s.chiba-u.jp/ishikawa/
• [Remarks] 千葉大学 大学院理学研究科 生物学教室 細胞生理学研究室
  • URL: http://life.s.chiba-u.jp/ishikawa/