Structural biology of Vesicular glutamate transporter

• Project/Area Number: 25840033
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Okayama University
• Principal Investigator: JUGE Narinobu 岡山大学, 自然生命科学研究支援センター, 助教 (60646917)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: VGLUT / グルタミン酸 / 構造生物学 / 小胞型グルタミン酸トランスポーター / 生化学 / 結晶構造解析

Outline of Final Research Achievements

In this project, I got the resolution data that is all most enough to publish. I am now making the article for this paper and I am now also making the better crystals. In the 2015, we will publish those results. And we also got the two papers in PNAS and J. Neurochem.. Those papers are related to inner membrane transporter that is same as vesicular glutamate transporter. Those data are also feedback to the analysis of Vesicular glutamate transporter.

Research Products

• [Journal Article] Wide expression of type I Na+/phosphate cotransporter 3 (NPT3/SLC17A2), a membrane potential-driven organic anion transporter. (2015)
  • Author(s): Togawa N., Juge N., Miyaji T., Hiasa M., Omote H., Moriyama Y.,
  • Journal Title: Am. J. Physol. Cell. Physiol. Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Plasmodium falciparum chloroquine resistance transporter is a H+-coupled polyspecific nutrient and drug exporter. (2015)
  • Author(s): Juge N, Moriyama S, Miyaji T, Kawakami M, Iwai H, Fukui T, Nelson N, Omote H, Moriyama Y
  • Journal Title: Proc Natl Acad Sci USA Volume: 112 Issue: 11 Pages: 3356-3361
  • DOI: 10.1073/pnas.1417102112
  • Peer Reviewed / Open Access
• [Journal Article] Vesicular GABA transporter transports beta-Alanine (2013)
  • Author(s): Narinobu juge, Hiroshi Omote, Yoshinori Moriyama
  • Journal Title: Journal of Neurochemistry Volume: 127 Pages: 482-486
  • Peer Reviewed
• [Presentation] マラリア原虫クロロキントランスポーター(pfCRT)の機能解析 (2015)
  • Author(s): 樹下成信
  • Organizer: 日本薬学会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] 小胞型GABAトランスポーターはbeta-アラニンを輸送する (2013)
  • Author(s): 樹下成信
  • Organizer: 日本生化学会中国四国支部会
  • Place of Presentation: 徳島大学
• [Presentation] 小胞型GABAトランスポーターはbeta-アラニンを輸送する (2013)
  • Author(s): 樹下成信
  • Organizer: 生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 東京大学