The structural insight into ROS suppression mechanism of type II NADH dehydrogenase (NDH-2)

Research Project

Project/Area Number	25840036
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Functional biochemistry
Research Institution	Kagawa University
Principal Investigator	YAMASHITA Tetsuo 香川大学, 医学部, 助教 (80444727)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords	活性酸素 / 反応機構 / フラボプロテイン / 基質結合部位 / NADH脱水素酵素 / ミトコンドリア / 電子伝達系 / エネルギー代謝 / 国際情報交換
Outline of Final Research Achievements	To elucidate the radical oxygen-suppression mechanism of mitochondrial type-II NADH dehydrogenase (NDH-2), we investigated biochemical properties of NDH-2 and its Thr239 mutant which have relatively high non-physiological NADH oxidase activity compared with wild type enzyme. By the kinetics, spectrographic and physicochemical analysis, it was strongly suggested that the formation of the charge-transfer complex between FAD-semiquinone, which is generated in the enzyme reaction process, and substrate NADH is responsible for the suppression of radical oxygen.

Report

(3 results)